2-pyrrolidone

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP study conducted according to national standard test guidelines

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38412 Part 9

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Strain: SAG 86.61
- Source (laboratory, culture collection): Algensammlung Goettingen

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test temperature:

24.8 °C

pH:

7.84 - 8.13

Nominal and measured concentrations:

Nominal: control, 25, 50, 100, 250, 500 mg/L

Reference substance (positive control):

not specified

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

22.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Based on the determined fluorescence values growth rate and yield were recalculated.

Treatment [mg/L]	Growth rate inhibition [%]	Yield Decrease [%]
Control	0	0
25	6.7	17.8
50	13.6	36.3
100	32.0	64.8
250	21.9	52.4
500	34.6	66.7

Concentration [mg/L]	pH values
	0 h	96 h
Control	7.84	7.92
25	7.87	7.99
50	7.89	8.04
100	7.86	8.07
250	7.89	8.12
500	7.88	8.13

Validity criteria fulfilled:

yes

Conclusions:

After an exposure time of 72 h following effect values were observed: EC10 = 22.2 mg/L; EC50 > 500 mg/L.

Executive summary:

Possible toxic effects of the test substance towards algae were investigated in a study which followed German standard DIN 38412 (Part 9). The following nominal concentrations: 25, 50, 100, 250, 500 mg/L and a control were investigated. After an exposure time of 72 h following effect values were observed: EC10 = 22.2 mg/L; EC50 > 500 mg/L.

Description of key information

The key values for chemical safety assessment are taken from the experimental result by BASF AG (1989).

Possible toxic effects of 2 -Pyrrolidone towards algae were investigated in a study which followed German standard DIN 38412 (Part 9). After an exposure time of 72 h following effect values were observed: EC10 = 22.2 mg/L; EC50 > 500 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:

500 mg/L

Additional information

The aquatic toxicity of 2 -pyrrolidone towards algae was experimentally investigated according to German standard DIN 38412 (Part 9); (BASF AG, 1989). The growth of Desmodesmus subspicatus as test organism was fluorimetrically determined. The test organisms were exposed to test concentrations of 0 (control), 25, 50, 100, 250, 500 mg/L mg/L for 72 hours. As final result, an EC10 (72 h) of 22.2 mg/L and an EC50 (72 h) of > 500 mg/L were determined.