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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable data which meets basic scientific principles, with acceptable restrictions (purity of the test substance not specified)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1961
Report Date:
1961

Materials and methods

Principles of method if other than guideline:
BASF-Test
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Physical state: solid
Degree of Purity: no data

Test animals

Species:
mouse
Strain:
not specified
Sex:
male/female
Details on test animals and environmental conditions:
Strain: Tuebinger
Breeder: BASF AG
Mean body weight for the males: ca. 30 g
Mean body weight of the females: ca. 25 g

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
water
Doses:
3200, 3600 and 4000 mg/kg bw
No. of animals per sex per dose:
5
Details on study design:
TEST PROCEDURE The study was conducted according to an internal BASF method. Several groups of 5-10 mice per sex and dose were treated intraperitoneally with preparations of the test substance as 20% aqueous solution (w/v). Each of the low and the high test dose group (i.e. 3200 and 4000 mg/kg bw) consisted of 5 animals, i.e. 2 males and 3 females); the mid dose group consisted of a total of 10 animals (5/sex). Each animal received a single intraperitoneal injection of test solution and the application volume ranged between 16 and 20 ml/kg bw. The animals were observed for mortality and for clinical symptoms of toxicity. At the end of the observation period of 7 days, the surviving animals were sacrificed for the purpose of necropsy; animals that died during the observation period also were subjected to necropsy. The LD50 value was estimated on the basis of the observed mortalities.

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
3 800 mg/kg bw
Mortality:
Mortality was 100% at the highest tested dose of 4000 mg/kg bw, and 10% at 3600 mg/kg bw (1 female died); the animals died within the first 24 hours following treatment. No mortality was seen in the 3200 mg/kg bw group.
Clinical signs:
A series of symtoms indicative of toxicity were reported for all groups. These symptoms mainly included accelerated breathing, convulsion or convulsive twitches, tonic-clonic spasms, prone position and apathy; they almost were observed within the first 30 minutes following injection. The surviving animals of the 3600 and 3200 mg/kg bw group recovered within one day.
Gross pathology:
Necropsy of the animals that died during the observation period revealed pale liver and kidney (fatty ?). No further abnormalities were seen. The survivors that were sacrificed at the end of the observation period of 7 days were inconspicuous.

Applicant's summary and conclusion