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EC number: 231-272-0
CAS number: 7473-98-5
O2 concentration Start [mg/L]
O2 concentration after 6 minutes [mg/L]
O2 consumption rate [mg/L in 6 min]
O2 consumption rate [mg/L*h]
Specific O2 consumption rate [mg O2/g*h]
RS/TS concentrations [mg/L]
Calculation of inhibition respiration [%]
Legend: TS = test item, RS = reference
item, BC = blank control
In this guideline (OECD 209) study with
GLP certification, the 3 hour EC50 of the test material to sewerage
microorganisms was determined to be >1000 mg/l (nominal). The test
was conducted upon a washed activated sewerage sludge taken from a
municipal sewerage treatment plant. The test material is not expected to
inhibit sewerage microorganisms at low concentrations.
Study conducted to recognised training guidelines with GLP certification.
In the key study on the toxicity of the
test substance on activated sludge an EC10 of 450 mg/L and an EC50 of >
1000 mg/L was reported. The test was conducted under GLP regulations and
according to OECD test guideline 209. The respiration inhibition was
determined in activated sludge obtained from a municipal sewage
treatment plant. The effect concentrations were derived after a test
duration of 3 hours.
A supporting study was conducted using the
test material before the final distillation and industrial activated
sludge. The EC50 after 30 min was determined to be 3 mg/L. However, the
value will not be used in the risk assessment since test material
without the final distillation was used and a more reliable up-to-date
key study is available.
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