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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Qualifier:
according to
Guideline:
other: German industrial standard test guideline DIN 38412, part 15
Deviations:
not specified
GLP compliance:
not specified
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: generation of a dispersion, test substance of low water solubility
Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Golden ide
- Source: Fa. Zoo Meyer, Pallaswiesenstr., Darmstadt, Germany
- Feeding during test: none
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20 °C
Nominal and measured concentrations:
Nominal: 100, 125, 150, 200 mg/L
Details on test conditions:
TEST SYSTEM
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 1
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
160 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Conclusions:
The test substance is with high probability not acutely harmful to fish.
Executive summary:

In this national guideline (DIN 38412, part 15) study conducted to generally accepted scientific standards, the 48 hour LD50 of the test material (EC 231-272-0) was determined to be 160 mg/l (nominal). The test was conducted on Golden ide fish under static conditions. The test result does not trigger classification and labelling of the test material under the criteria of the EU Classification, Labelling, & Packaging (CLP) regulation (1272/2008).

Description of key information

 Test procedure in accordance with national standard methods with acceptable restrictions.

Key value for chemical safety assessment

LC50 for freshwater fish:
160 mg/L

Additional information

In a toxicity study on fish according to the national standard test guideline DIN 38412 part 15 (Merck, 1989), the toxicity of the test item was determined. Golden ide was used as test organism and the LC50 (48 h) determined in the test was 160 mg/L. Even though material without the final distillation step was used it can be used as weight of evidence since the LC50 is above 100 mg/L and algae and daphnids are the more sensitive species. Algae are the most sensitive and, therefore, the result of the algae test will trigger the risk assessment.

Additionally, the risk assessment has been conducted with a Risk Characterization Ratio below 1. Hence, all relevant uses of the test substance are considered to be safe. Therefore, and due to animal welfare reasons it is not justified to conduct an additional fish test.