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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not specified
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
- Sample storage conditions before analysis: Samples were diluted with isopropanol to a total volume of 100 mL. Aliquots were filtered and used for GC/MS-analysis
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was weighed in (119.92 mg) and made up with 1000 mL M4 medium. The mixture was stirred on a magnetic stir plate for about 20 minutes (under light exclusion)
- Differential loading: no
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no remarkable observations, clear test medium
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Great water flea
- Source: The clone was originally supplied by the Institut National de Recherche Chimique Appliquée, France in 1978. From this date on this clone was cultured and bred continuously in the laboratory of Ecotoxicology of the Experimental Toxicologx and Ecology of BASF SE in Ludwigshafen
- Age at study initiation (mean and range, SD): < 24 hours (at least 3rd brood progeny)

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not): In synthetic fresh water (Elendt M4) prepared according to OECD 202 at 20 ± 1 °C. Photoperiod: 16 hours light, 8 hours dark. Culture water was renewed twice a week.
- Type and amount of food: Unicellular algae Desmodesmus subspicatus, cultured continuously at the test facility
- Feeding frequency: daily
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
2.2 - 3.2 mM
Test temperature:
19.4 - 19.8 °C
pH:
8.2
Dissolved oxygen:
8.8 mg/L
Nominal and measured concentrations:
nominal: control, 120 mg/L
mean measured: 119.3 mg/L (99.4% recovery)
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass beakers (nominal volume 100 mL)
- Material, size, headspace, fill volume: fill volume = 50 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 according to OECD guideline 202
- Ca/Mg ratio: about 4:1
- Conductivity: 550 - 650 µS/cm
- Intervals of water quality measurement: temperature was measured continuously, pH and oxygen content at test start and end

OTHER TEST CONDITIONS
- Photoperiod: in darkness (test substance sensitive to light)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility

TEST CONCENTRATIONS
- Range finding study: 100 mg/L did not cause any immobilisation
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study: EC50 > 100 mg/L
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 119 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 119 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
- Results with reference substance valid? yes
- EC50/LC50: 0.67 mg/L
Validity criteria fulfilled:
yes
Conclusions:
The test substance is with high probability not acutely harmful to aquatic invertebrates.
Executive summary:

In this guideline (OECD 202) study, the 48 hour EC50 of the test material (EC 231 -272 -0) to Daphnia magna was determined to be >119 mg/l (nominal). The test was conducted under static conditions. The result of the this test is not sufficient to trigger classification and labelling under the EU Classification, Labelling, and Packaging (CLP) regulation (1272/2008).

Description of key information

 Study conducted to recognised training guidelines with GLP.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
119 mg/L

Additional information

In a limit test conducted according to OECD 202 guideline (BASF SE, 2010) an EC50 of > 119 mg/L based on mean measured concentrations was determined. At this single test substance concentration no immobile daphnids were observed, hence the EC0 was > 119 mg/L as well.