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EC number: 231-272-0
CAS number: 7473-98-5
In this guidleine (OECD 201) study
conducted with GLP certification, the 72 hour EC50 of the test material
EC 231-272-0 to algae ( Desmodesmus subspicatus) was determined to be
1.95 mg/l. The test was conducted in freshwater, static, conditions.
Test concentrations ranged from 0.1 to 10 mg/l (nominal). As the 72 hour
EC50 is >1 mg/l and <10 mg/l, this result would normally trigger
classification of the test material as hazardous to the aquatic
environment according to CLP 1272/2008 Chronic Category 2. However, as the
substance is readily biodegradable, the classification is not triggered.
Study conducted to recognised training guidelines with GLP certification
key study for the determination of the inhibition of algal growth was
conducted according to OECD guideline 201 including analytical test item
concentration verification (BASF SE, 2010). The ErC50 (72 h) was 1.95
mg/L based on the geometrical mean measured test item concentration. The
ErC10 was determined to be 0.629 mg/L. Therefore, the test susbstance is
acutely toxic to aquatic algae.
supporting study was conducted (ABC Laboratories, Inc., 1993).
algae toxicity test followed only standard procedures used in the
testing laboratory, no official guideline is mentioned. However, as
indicated by the test design, the test procedure may be considered
similar to a OECD 201 test. Nevertheless, the test was conducted without
analytical monitoring and the section-by-section specific growth rates
are not given. The EC50 after 72 h based on cell counts was determined
to be 0.64 mg/L. Due to the deficiencies the values are not used in the
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