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EC number: 231-272-0
CAS number: 7473-98-5
Tab. 1: Results
Duration of the adaptation phase:
Degree of Biodegradation of the test substance at the end of the ten-day window
about 90 % CO2/ThCO2
Degree of Degradation DOC of the test
substance at the end of exposure
90-100 % DOC removal
Degree of Biodegradation of the test substance at the end of exposure, mean value
90-100 % CO2/ThCO2
Degree of Biodegradation of the reference substance after 14 days % CO2/ThCO2
92 % CO2/ThCO2
Degree of Biodegradation in the inhibition control after 14 days
76 (% CO2/ThCO2)
substance assays and the inhibition control assay were acidified by
adding 2 mL of concentrated hydrochloric acid after 28 days of exposure.
The blank control- and reference substance assays were not acidified,
since they were still required for other ongoing studies.
amounts of released carbon dioxide in the traps of the test assays both
with and without acidification were determined on day 29 and added to
the amount calculated from day 28.
required pass level for the assessment of the test substance as ready
biodegradable was reached at day 19 after start of exposure. Therefore
the abandonment of the acidification of the blank control- and reference
substance assays had no influence upon the evaluation of the test result.
Theoretical amount of formed Carbon dioxide,
= Dissolved Inorganic Carbon
In this guideline (OECD 301 B) study
conducted with GLP certification, the test material (EC 231-272-0) was
determined to be readily biodegradable
Study conducted to recognised training guidelines with GLP
In 2010 BASF SE conducted a ready
biodegradability test for the test susbtance according to OECD 301 B
guidelines under GLP regulations. Evolving CO2 was captured in sodium
hydroxide solution and TIC was measured at least twice a week. After a
lag phase of approximately 9 days the degradation degree reached about
90% at the end of the 10 -day window. Therefore, the test substance is
readily biodegradable according
to OECD criteria.
supporting study according to EU Method C.6 (Degradation: Chemical
Oxygen Demand) was conducted using the test material before the final
distillation (Merck, 1989). After 28d a biodegradation of 59% was
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