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EC number: 200-927-2
CAS number: 76-03-9
The NOEL value at 21 and at 35 days wqas equal or greater than 2500
A spermhead morphology assay was performed in mice with
sodium trichloroacetate. B6C3F1 mice (24/group) were given 0, 625, 1250,
or 2500 mg/kg bw/day of test substance for five consecutive days by oral
gavage. Twelve mice per treatment group were sacrificed at 21 and 35
days after the first treatment. These sacrifice times correspond to
treatment of cells at the late spermatogonial and primary spermatocyte
periods, respectively, assuming no treatment-related delays in sperm
development. Body weights were recorded for each animal during the
dosing phase and at sacrifice, and testes and cauda epididymidal weights
were recorded at sacrifice. Ten animals from each treatment/sacrifice
group were randomly selected to be evaluated for sperm count and
morphology. The types and frequencies of spermhead abnormalities were
based on the scoring of 500 sperm per animal. Initial and sacrifice body
weights, testes and cauda epididymal weights, testes to final body
weight ratios, and sperm concentration/mg of cauda epididymis were
analyzed by one-way analysis of variance (ANOVA) at a significance level
of p < 0.05. When significant differences were found, Dunnett's test was
applied to compare the different treatment group means with the negative
control means. The numbers of morphologically abnormal sperm were
analyzed by the Kruskall-Wallis and Mann-Whitney U tests, with
differences considered significant at the p < 0.05 level. These latter
tests are nonparametric alternatives to ANOVA and Dunnett's test,
The testes-to-final-body weight ratio, the number of
sperm per milligram of cauda epididymis, and the percentage of abnormal
spermheads were unaffected in the treated animals either at 21 or 35
days following oral gavage administration.
The NOEL value at 21 and at 35 days was equal or greater
than 2500 mg/kg bw/day.
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