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EC number: 200-927-2 | CAS number: 76-03-9
Toxicity to aquatic plants other than algae
Administrative data
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Test method was similar to OECD guideline 221. However, no data on analytical monitoring. No data on GLP.
Data source
Reference
- Reference Type:
- publication
- Title:
- Haloacetic acids in the aquatic environment. Part I: macrophyte toxicity.
- Author:
- Hanson ML, Solomon KR
- Year:
- 2 004
- Bibliographic source:
- Environmental Pollution 130 (2004) 371-383
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- Deviations:
- not specified
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Trichloroacetic acid
- EC Number:
- 200-927-2
- EC Name:
- Trichloroacetic acid
- Cas Number:
- 76-03-9
- Molecular formula:
- C2HCl3O2
- IUPAC Name:
- trichloroacetic acid
- Details on test material:
- - Name of test material (as cited in study report): TCA
- Analytical purity: 99.7 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The acid solutions were titrated to the required growth media pH with sodium hydroxide.
Test organisms
- Test organisms (species):
- Lemna gibba
- Details on test organisms:
- L. gibba was cultured axenically according to the methods outlined in Greenberg et al. (1992) with Hunter’s media containing 10 g/L sucrose. Culturing was conducted in a laminar flowhood.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
Test conditions
- Test temperature:
- 25 ± 2 ºC
- Nominal and measured concentrations:
- Nominal concentrations: 3, 10, 30, 100, 200, 400, and 800 mg/L.
- Details on test conditions:
- Approximately 10 days prior to a L. gibba toxicity test, plants were transferred from growth media containing sucrose to media without sucrose. This
was done so that the plants would switch from heterotrophic to autotrophic energy production.
L. gibba testing was conducted according to the methods outlined in Greenberg et al. (1992) with growth chamber conditions maintained under standard conditions for this test.
The growth chamber was a model E7H Controlled Environments chamber.
Light was maintained for 24 h at approximately 6200 lux.
Each test solution was transferred to a 10-mL plastic Petri dish (Fisher Scientific) and two plants, each with four fronds, for a total of eight fronds, were introduced and monitored.
Test solutions were changed on day 3 and 5 to maintain consistent levels of the compound under study.
- Control: n=6
- Exposed plants: n=3
The endpoints monitored were frond number, colony number, wet mass, frond mass, frond growth rate and chlorophyll a, chlorophyll b, and total chlorophyll concentrations. Pigment concentrations were determined simultaneously by extraction in ACS grade N,N'- dimethyl formamide (Fisher Scientific, Fair Lawn, NJ, USA) using the method and calculations outlined by Porra et al. (1989) and measured with a PU8620 Series UV/visible Spectrophotometer (Philips Analytical, Natick, MA, USA) on a fresh-weight basis. - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 864.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: (95% CL = 575.2-1153.3)
- Duration:
- 7 d
- Dose descriptor:
- other: EC25
- Effect conc.:
- 215.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: (95% CL = 129.6-302.0)
- Duration:
- 7 d
- Dose descriptor:
- EC10
- Effect conc.:
- 53.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: (95% CL = 10.8-96.9)
- Details on results:
- The most sensitive endpoint was the Frond number and pigments was the least sensitive endpoint.
Any other information on results incl. tables
The EC50 was 864.3 (575.2 -1153.3) mg/L (basis for effect: growth rate).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Remarks:
- (No data on control results)
- Conclusions:
- The EC50 was 864.3 (575.2 -1153.3) mg/L (basis for effect: growth rate).
- Executive summary:
Laboratory tests were conducted with the macrophyte Lemna gibba to assess the toxicity of Trichloroacetic acid.
The EC50 was 864.3 (575.2 -1153.3) mg/L (basis for effect: growth rate).
The most sensitive endpoint was the Frond number and pigments was the least sensitive endpoint.
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