Trichloroacetic acid

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The species is not the recommended one for classification and the observation period was shorter than the period recommended in the guidelines.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1941

Materials and methods

Principles of method if other than guideline:

The acute toxicity was determined for trichloroacetic acid. The mice used were young adult Albinos of a heterogeneous stock with the sexes equally distributed. Test solution was administered orally by a straight metal catheter attached to a syringe. The doses tested were 3162, 3981, 4217, 4467, 4500, 4732, 5012, 5309, 5623 and 6000 mg/kg. Immediately following the treatment the animals were observed for 6 days.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Trichloroacetic acid

EC Number:

200-927-2

EC Name:

Trichloroacetic acid

Cas Number:

76-03-9

Molecular formula:

C2HCl3O2

IUPAC Name:

trichloroacetic acid

Details on test material:

- Name of test material (as cited in study report): trichloroacetic acid

Test animals

Species:

mouse

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: Young adult

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

The acid solution before administration was adjusted with sodium hydroxide to a range of pH between 6 and 7.
Volume of administration: approximately 0.01 cc. of solution per gram of body weight.

Doses:

3162, 3981, 4217, 4467, 4500, 4732, 5012, 5309, 5623 and 6000 mg/kg

No. of animals per sex per dose:

10 animals per dose (5 males and 5 females)

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 6 days
- Other examinations performed: clinical signs, body weight, behaviour.

Statistics:

The dosage-mortality curves were constructed according to the method of Bliss.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

4 970 mg/kg bw

95% CL:

4 700 - 5 260

Mortality:

Table of results: Doses of acid administered and numbers of fatalities resulting from each dose.

Clinical signs:

other: Animals receiving trichloracetic acid quickly passed into a state of narcosis or seminarcosis, and within 36 hours either recovered completely or died without coming out of the narcosis. The intoxication symptoms which occurred in mice included ataxia, se

Any other information on results incl. tables

Table 07.02.01_01. Doses administered and numbers of fatalities resulting from each dose.

 

Dose (mg/kg)	Mortality rate
3162	0/10
3981	1/10
4217	1/10
4467	2/10
4500	7/10
4732	5/10
5012	3/10
5309	4/10
5623	7/10
6000	10/10

 

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The LD50 of trichloroacetic acid for mice was 4970 mg/kg (the acid solution before administration was adjusted with sodium hydroxide to a range of pH between 6 and 7).

Executive summary:

The acute toxicity was determined for trichloroacetic acid. The mice used were young adult Albinos of a heterogeneous stock with the sexes equally distributed. Test solution was administered orally by a straight metal catheter attached to a syringe. The doses tested were 3162, 3981, 4217, 4467, 4500, 4732, 5012, 5309, 5623 and 6000 mg/kg. Immediately following the treatment the animals were observed for 6 days. The dosage-mortality curves are constructed for each substance according to the methods of Bliss.

The LD50 of trichloroacetic acid for mice was 4970 mg/kg. Depending on the dosage strength, the experimental animals died between two hours and, at the least, three days after oral application of lethal doses. The intoxication symptoms which occurred in mice included ataxia, sedation, prone and lateral position, diminished food uptake, decreased capability of triggering reflexes, palsy of the hind extremities and coma.