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EC number: 231-955-3
CAS number: 7782-42-5
Summary table of results
Limit test with
Wistar WI (WU) rats
Aerosol concentration (mg/m3) - mean (SD)
a aerosol photometer (n=5)
b filter samples (n=5)
3.0 µm (n=2)
Number of exposed rats
Exposure duration (hrs)
Number of rats showing affected reflexes 1/24/48 hrs after end of exposure
0 / 0 / 0
Body temperature decrease measured after end of exposure Δ° C: mean (SD)
Number of dead ani-mals within 14-day post-exposure period
Rats showing abnor-malities in gross pa-thology on day 14 of post-exposure period
different compared to controls * p<0.05 ** p<0.01 *** p<0.001 (Student’s
t-test); n = number of measurement
and Controlling of the Exposure Atmosphere
flow, temperature and relative humidity were measured continuously and
recorded as 10-minute means. Exposure concentrations were measured
continuously by an aerosol photometer (by 10-minute intervals). In
addition, they were controlled by taking filter samples (5 times per
4-hour inhalation). The MMAD of the aerosol phase was determined twice
within the 4-hour exposure period using a cascade impactor (Marple
impactor) with subsequent gravimetrical analysis. Measurements during
exposure on Jan 27, 2010 resulted in the following data:
x 103+0.06 x 103mg/m3 (n=5)
aerosol concentration of 2.02
x 103+0.06 x 103mg/m3,
determined by the aerosol photometer, is an optimum for an acute study
as guideline OECD 403 concedes a standard deviation of up to 20%. The
gravimetrically determined concentrations are within this range as well.
· MMAD =
2.94 µm ; geometric SD = 2.04
· MMAD =
3.12 µm ; geometric SD = 1.97 Mean:3.03
µm (0.09) (n=2)
values are approx. 3 µm; this threshold should not be exceeded in order
to ensure a sufficient respirability of the aerosols (OECD guideline 403
recommendations allow the following ranges: MMAD 1-4 µm; geometric SD =
1.5 - 3).
an acute nose-only inhalation study (limit test) 3 male and 3 female
rats were exposed for 4 hours to a target concentration of 2000 mg Expanded
Graphite Powder per m3
animal welfare and technical reasons, i.e. to prevent agglomeration of
particles and to assure an MMAD in the respirable range from 1 -4μm,
an aerosol concentration of 2000 mg test item/ m3 was
used in this Limit Test, as recommended by Regulation (EC) No. 1272/2008
and OECD guideline 403.
cessation of exposure none of the rats exposed to the test item showed
any signs of toxicity. Only usual signs of discomfort after exposure to
particles were observed. Grooming activity started immediately after the
end of exposure.
temperature was statiscally significantly decreased by 2.5 °C in the
female group, however, not in the male group. Such effect is commonly
observed after exposure to large aerosol concentrations indicating a
reduction of body functions and reversible.
6 rats showed a good general health status during the subsequent 14 -day
postexposure observation period.
6 rats did not show any test item-related abnormalities upon necropsy.
the results observed in this acute 4 -hour nose-only inhalation study
the following classification was derived for the test item Expanded
Graphite Powder: Unclassified
(according to Regulation (EC) No. 1272/2008)
not determined in this study. An experimentally determined LC50
would be higher than
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