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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The short term fish toxicity was determined to be > 100 mg/L for the LC50 and > 100 mg/L for the NOEC, following Testing guidelines OECD 201 as well as EU C1

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

The results for the LC50 and the NOEC (both > 100 mg/L) are based on the nominal loading of the test item. Since it is not possible to detect the substance graphite in the test solution, no other value than the loading amount is available. Due to the poor water solubility oft he test item the medium for the fish test was prepared according to OECD Guidance 23 "GUIDANCE DOCUMENT ON AQUATIC TOXICITY TESTING OF DIFFICULT SUBSTANCES AND MIXTURES" as water accommodated fraction (WAF).

Although the substance graphite itself appears not to be bioavailable, toxic effects cannot be excluded, because of the unknown influence of its impurities. This is why the test item used for the acute fish test consist of a mixture of two different graphite qualities, representing a typical spectrum of graphite's impurities. In the limit test conducted, no significant signs of toxicity were observed. In conclusion neither the material graphite itself nor its impurities reached concentrations where aquatoxic effects occur.