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EC number: 283-294-5
CAS number: 84604-16-0
Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Saccharomyces cerevisiae, Saccharomycelaceae.
Biodegradation on various days during the 28-day period
of incubation :
The Yeast extract, Saccharomyces cerevisiae,
(SPRINGER 0251 / 0-MG-L) was tested for ready biodegradability according
to the OECD guideline No. 310, 2006, and ISO
International Standard 14593, 1999, and following the GLP. This study
was requested by ECHA in the decision CCH-D-0000004821
The tested substance was an UVCB, easily soluble in mineral
medium. No correction was made for the purity/composition of the test
substance. In a pre-test the TOC
concentration of 5 g Yeast extract/l was determined to be
2040 mg/l. This value was used to set the concentration in the main
In the main test, Yeast extract was added to mineral medium to
give a final organic carbon concentration of 20 mg C/l. The test media
were prepared using a 100-times concentrated stock solution, a clear
yellow stock solution of 5 g Yeast extract/l. Volumes of 1.07 ml of this
stock solution were added to mineral medium inoculated with a mixed
population of micro-organisms (final volume 107 ml). The test was
performed in sealed bottles with a headspace of air.
The test consisted of four groups:
The CO2 evolution resulting from the aerobic
biodegradation of the test substance was determined by measuring the
inorganic carbon (IC) produced in the test bottles in excess of that
produced in blank vessels containing inoculated medium only. The extent
of biodegradation was expressed as a percentage of the theoretical
maximum IC production, based on the quantity of test substance initially
added (as organic carbon).
The relative biodegradation values calculated from the
measurements performed during the test period revealed 72%
biodegradation of Yeast extract at the end of the test. Furthermore,
biodegradation of Yeast extract of at least 60% was reached within a
10-day window. In the toxicity control,Yeast
extractwas found not to inhibit
microbial activity. Since all criteria for acceptability of
the test were met, this study was considered to be valid.
In conclusion, Yeast extract is designated as readily
biodegradable, fulfilling the 10-day window.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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