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EC number: 283-294-5
CAS number: 84604-16-0
Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Saccharomyces cerevisiae, Saccharomycelaceae.
ANALYSIS OF CARBON CONTENT
carbon contents of the renewal periods (24 h)
Nominal conc. 100 mg/L Test Item (WAF)
Measured carbon content
at the start
Measured carbon content at the end
First renewal period
15.2 ± 5.21
15.3 ± 3.06
Second renewal period
16.5 ± 1.39
16.4 ± 1.00
of the regression lines
Date of measurement
12 March 2018
13 March 2018
14 March 2018
The acute toxicity of Saccharomyces cerevisiae lysate to Daphnia magna
was determined according to EU Method C.2 and OECD 202, and in
compliance with GLP.
20 daphnids distributed in 4 vessels were exposed to the test item in a
semi-static test during 48 hours. In a preliminary formulation study,
the optimal conditions for the preparation of the Water Accomodated
Fraction (WAF) were determined. The highest carbon content at 20°C was
obtained after 0.5h hour of stirring of a 100 mg/L test item loading
rate, followed by filtration through a fine (0.22 μm) filter to give the
100% saturated solutions (WAF). Since no toxicity was observed during
the range-finding study, a limit test at the loading rate of 100 mg/L
test item (WAF solution) was performed.
Since the test item is an UVCB, with a very complex composition and
there is no single chemical marker that can be readily analysed, the
non-purgeable organic carbon (NPOC) method was used to control the
tested concentration. The concentration was measured at start and at the
end of the renewal periods (from an additional vessel without the test
organisms which would affect the total carbon present in solution). The
mean measured concentration was 15.9 ± 0.6 mg carbon /L and was stable
during the exposure periods.
No immobilisation was observed during the study at the loading rate of
100 mg/L test item (WAF solution). There was no immobilized animal in
the control group and the dissolved oxygen concentration at the end of
the test in control and test vessels was more than 3 mg/L. All validity
criteria were within acceptable limits.
The 48h-EL50 to Daphnia magna of Saccharomyces cerevisaie lysate is
superior to 100 mg/L (WAF solution) and the NOELR is equal to 100 mg/L
(WAF solution), which corresponds to 15.9 mg/L carbon content.
This acute toxicity to Daphnia magna is classified as acceptable, and
satisfies the guideline requirements of EU Method C.2 and OECD 202.
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