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EC number: 283-294-5
CAS number: 84604-16-0
Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Saccharomyces cerevisiae, Saccharomycelaceae.
ANALYSIS OF CARBON CONTENT
carbon contents of the renewal periods (24 h)
Nominal conc. 100 mg/L Test Item (WAF)
Measured carbon content
at the start
Measured carbon content at the end
First renewal period
17.4 ± 4.72
17.8 ± 1.58
Fourth renewal period
17.4 ± 1.08
17.2 ± 0.483
of the regression lines
Date of measurement
12 March 2018
13 March 2018
15 March 2018
16 March 2018
The acute toxicity of Saccharomyces cerevisiae lysate to fish was
determined according to EU method C.1 and OECD 203, and in compliance
Brachydanio rerio was exposed to the test item in a semi-static test
during 96 hours. In a preliminary formulation study, the optimal
conditions for the preparation of the Water Accomodated Fraction (WAF)
were determined. The highest carbon content at 20°C was obtained after 1
hour of stirring of a 100 mg/L test item, loading rate followed by
filtration through a fine (0.22 µm) filter to give the 100% saturated
solutions (WAF). Since no toxicity was observed during the range-finding
study, a limit test at the loading rate of 100 mg/L test item (WAF
solution) was performed.
Since the test item is an UVCB, with a very complex composition and ther
is no single chemical marker that can be readily analysed, the
non-purgeable organic carbon (NPOC) method was used to control the
tested concentration. The concentration was measured at start and at the
end of the first and last renewal periods (from an additional vessel
without the test organism which would affect the total carbon present in
solution). The mean measured concentration was 17.5 mg carbon /L and was
stable during the exposure periods.
No mortality or any sub-lethal effect was observed during the study at
the loading rate of 100 mg/L test item (WAF solution). No mortality
occurred in the control and constant conditions were maintained during
this test with temperature between 20.3–21.3°C, pH between 7.24–7.73 and
dissolved oxygen ≥ 78%.
The 96h-LL50 to fish of Saccharomyces cerevisaie lysate is superior to
100 mg/L (WAF solution) and the NOELR is equal to 100 mg/L (WAF
solution), which correspond to 17.5 mg/L carbon content.
This acute toxicity to fish is classified as acceptable, and satisfies
the guideline requirements of EU Method C.1 and OECD 203.
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