Registration Dossier

Administrative data

Description of key information

Key value for chemical safety assessment

Skin sensitisation

Endpoint conclusion
Endpoint conclusion:
adverse effect observed (sensitising)
Additional information:

In a Murine Local Lymph Node Assay (LLNA) according to OECD 429, 4 -Nitrotoluene-2 -sulphonic acid shows a skin sensitizing effect. No signs of systemic toxicity were noticed (BASF SE, 2010).

When applied as 40% preparation in propylene glycol, the test substance induced a biologically relevant response (increase to 1.5 fold or above of control value = stimulation Index (SI) >=1.5) in the auricular lymph node cell counts. There was a relevant increase in lymph node weights, as well.Concomitantly, the increase of ³H-thymidine incorporation into the cells was biologically relevant (increase above the cut off stimulation index of 3) at this concentration.

The test-substance preparations did not cause any increase in ear weights.


Migrated from Short description of key information:
4-Nitrotoluene-2-sulphonic acid showed a skin sensitizing effect in the Murine Local Lymph Node Assay.

Justification for classification or non-classification

EU classification according to Annex VI of the Directive 67/548/EEC:

skin sensitizing, R43

GHS classification (EU GHS, CLP 1272/2008)

Skin sens. Cat. 1B