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EC number: 204-445-3
CAS number: 121-03-9
In-vitro Studies: Bacterial systems
In an Ames-Test (GINC, Japan) a dose-dependent tendency in the increase
of mutated colonies was observed in strain TA100, TA 98 and TA1537
without S9 mix and in strain TA100 with S9 mix. In the trials which were
repeated twice, the number of mutated colonies in the TA1535 group was
1.5 to 2 times higher than that of the solvent control, but it could not
be determined to be definitely positive. A confirmative test was carried
out with regard to the TA1535 strains with and without S9 mix to confirm
its reproducibility, but a clear mutagenicity-inducing effect was not
observed. On the other hand, the positive control substance induced two
times or more of mutated colonies in each tester strain, compared to the
solvent control group. In addition, remarkable changes such as
precipitation were not observed during the tests.
In-vitro Studies: Mammalian cell gene mutation test
An HPRT Locus Assay (BASF SE, 2010) with CHO cells was performed with
4-nitrotoluene-2-sulphonic acid. In the study the test substance did not
lead to a relevant increase in the number of mutant colonies either
without S9 mix or after the addition of a metabolizing system in two
experiments performed independently of each other. The mutant
frequencies at any concentration were within or slightly above the range
of the concurrent negative control values and within the range of the
historical negative control data.
In-vitro studies: Chromosomal aberrration and micronucleus tests
In the in vitro Chromosomal Aberration Test in Chinese hamster cells
with 4-nitrotoluene-2 -sulphonic acid, no tendency of inducing the
chromosomal structural aberration and ploidy cells was observed in
either dose (GINC, Japan). On the other hand, a remakable induction of
chromosomal structural aberrration was recognized in cells treated with
MMC (positive control). In case of the test substance group, no tendency
of inducing the chromosomal structural aberrration and ploidy cells was
observed in either dose in the absence and presence of S9 mix. On the
other hand, a remarkable induction of chromosomal structural aberration
was recognized only in the presence of S9 mix for the cells treted with
CP (positive crontrol). In each test system of continous treatment and
shortterm treatment, a decrease in pH value for the culture fluid was
recognized in all test doses, it has returned to the neutral range at
the exchange of culture fluid or the completion of culture.
EU classification according to Annex VI of the Directive 67/548/EEC:
no classification required
GHS classification (EU GHS, CLP 1272/2008):
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