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EC number: 204-445-3
CAS number: 121-03-9
Due to in vitro test results 4-nitrotoluene-2-sulphonic acid is considered to bi irritating to the skin and to cause serious damage to eyes.
Skin irritation /corrosion
4-nitrotoluene-2-sulphonic acid was irritating to the skin in the
Epiderm TM Skin Corrosion/Irritation Test (OECD 431) (BASF SE, 2010).
For the corrosion test the samples were incubated with the test
substance for 3 minutes and 1 hour, for the irritation test the samples
were incubated with the test substance for 1 hour followed by a 42
-hours post-incubation period. Tissue destruction was determined by
measuring the metabolic acitvity of the tissue after
exposure/post-incubation using a colorimetric test. The reduction of
mitochondrial dehydrogenase activity, measured by reducted formazan
production after incubation with a tetrazolium salt (MTT) was chosen as
The mean viability of the test substance treated tissues determined
after an exposure period of 3 minutes was 101%, and it was 17% after an
exposure period of 1 hour.The mean viability of the test-substnce
treated tissues determined after an exposure period of 1 hour with about
42 hours post-incubation was 7%.
4-nitrotoluene-2-sulphonic acid causes serious eye damage in the Bovine
corneal Opacity and Permeabilit Tet (OECD 437) (BASF SE, 2010). Corneal
opacity was measured quantitatively as the amount of light transmission
through the cornea. Permeability was measured quantitatively as the
amount of sodium fluorescein dye that passes across the full thickness
of the cornea. The mean Opacity change of the test-substance treted
corneas was 143.4. The mean OD490 of the test-substance treateed corneas
determined as permeability parameter was 0.013. The In Vitro Irritancy
Score (IVIS) of the test-substance treated corneas was calculated to be
EU classification according to Annex VI of the Directive 67/548/EEC:
- risk of serious damage to eyes, R 41
- irritating to skin, R 38
GHS classification (EU GHS, CLP 1272/2008)
- serious eye damage, category 1
- skin irritation, Category 2
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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