Sodium N-chlorobenzenesulphonamide

Additional information on environmental fate and behaviour

Administrative data

Endpoint:

additional information on environmental fate and behaviour

Type of information:

experimental study

Adequacy of study:

key study

Study period:

January to May 2013

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted non-GLP. A testing guideline was not applied.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

Solutions of the test material at different concentrations were exposed under "clean" and "dirty" conditions for various times. At various intervals, samples were taken and submitted to chemical analysis in order to determine the decompositoin (dechlorination) of the substance. Dirty conditions simulated conditions under which disinfection is appliacalbe. They furthermore are equivalent to methods for efficacy testing.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Sodium N-chlorobenzenesulphonamide

EC Number:

204-847-9

EC Name:

Sodium N-chlorobenzenesulphonamide

Cas Number:

127-52-6

Molecular formula:

C6H6ClNO2S.Na

IUPAC Name:

sodium chloro(phenylsulfonyl)azanide

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): Chloramine B
- Lot/batch No.: 050125
-Packaging: PE dose 1000 g

Results and discussion

Any other information on results incl. tables

The following results were obtained:

Chloramin B (BCHA) 0,01g/L, clean conditions

Time of analyse [hour]	Start	0,5	2	5	8	21	23	30	46	51	95	97
Content of BCHA in g/L	0,0115	0,0090	0,0075	0,0062	0,0054	0,0031	0,0028	0,0024	0,0014	0,0013	0,0003	0,0002
Content of BCHA (%)	100	78,3	65,2	53,9	47,0	27,0	24,3	20,9	12,2	11,3	2,6	1,7

Chloramin B (BCHA) 0,01g/L, dirty conditions

Time of analyse [hour]	Start	0,5	2	5
Content of BCHA in g/L	0,0104	0,0002	< 0,0002	no det.
Content of BCHA (%)	100	2,7	< 2,7

Chloramin B (BCHA) 0,1g/L, clean conditions

Time of analyse [hour]	Start	0,5	2	6	8	22	26	71
Content of BCHA in g/L	0,1050	0,0994	0,0932	0,0865	0,0845	0,0683	0,0693	0,0484
Content of BCHA (%)	100	94,6	88,7	82,3	80,4	65,0	66,0	46,1

Chloramin B (BCHA) 0,1g/L, dirty conditions

Time of analyse [hour]	Start	0,5	2	6	8	14	18	26	71
Content of BCHA in g/L	0,1041	0,0705	0,0551	0,0360	0,0309	0,0094	0,0025	0,0023	< 0,0002
Content of BCHA (%)	100	67,7	52,9	34,6	29,7	9,0	2,4	2,2	< 0,2

Chloramin B (BCHA) 1g/L, clean conditions

Time of analyse [hour]	Start	0,5	2	6	8	19	29	92	124	29d
Content of BCHA in g/L	1,0296		1,0296		1,0025	1,0025	1,0025	1,0025	1,0025	0,8229
Content of BCHA (%)	100		100		97,4	97,4	97,4	97,4	97,4	79,9

Chloramin B (BCHA) 1g/L, dirty conditions

Time of analyse [hour]	Start	0,5	2	6	8	19	29	92	124	29d
Content of BCHA in g/L	1,0296		0,9212		0,8213	0,7315	0,7044	0,6232	0,5419	0,3238
Content of BCHA (%)	100		89,5		79,8	71,0	68,4	60,5	52,6	31,4

Chloramin B (BCHA) 10g/L, clean conditions

Time of analyse [hour]	Start	0,5	2	5	11	24	27	31	48	72	9 d	38 d
Content of BCHA in g/L	10,162		10,162	10,162	10,162	10,162	10,162	10,162	10,162	10,162	9,982	9,131
Content of BCHA (%)	100		100	100	100	100	100	100	100	100	98,2	89,9

Chloramin B (BCHA) 10g/L, dirty conditions

Time of analyse [hour]	Start	0,5	2	5	11	24	27	31	48	72	38 d
Content of BCHA in g/L	10,162		10,117	10,072	10,027	9,937	9,937	9,891	9,801	9,666	8,636
Content of BCHA (%)	100		99,6	99,1	98,7	97,8	97,8	97,3	96,4	95,1	85,0

Chloramin B (BCHA) 20g/L, clean conditions

Time of analyse [hour]	Start	0,5	2	5	11	24	27	31	48	72
Content of BCHA in g/L	20,086		20,086	20,086	20,086	20,086	20,086	20,086	20,086	20,086
Content of BCHA (%)	100		100	100	100	100	100	100	100	100

Chloramin B (BCHA) 20g/L, dirty conditions

Time of analyse [hour]	Start	0,5	2	5	11	24	27	31	48	72
Content of BCHA in g/L	20,086		20,074	20,069	20,022	20,001	19,998	19,974	19,901	19,889
Content of BCHA (%)	100		99,9	99,9	99,7	99,6	99,6	99,4	99,1	99,0

Chloramin B (BCHA) 50g/L, clean conditions

Time of analyse [hour]	Start	0,5	2	5	11	24	27	31	48	72
Content of BCHA in g/L	49,998		49,998	49,998	49,998	49,998	49,998	49,998	49,998	49,998
Content of BCHA (%)	100		100	100	100	100	100	100	100	100

Chloramin B (BCHA) 50g/L, dirty conditions

Time of analyse [hour]	Start	0,5	2	5	11	24	27	31	48	72
Content of BCHA in g/L	49,998		49,997	49,991	49,876	49,801	49,706	49,665	49,611	49,599
Content of BCHA (%)	100		100	100	99,8	99,6	99,4	99,3	99,2	99,2

Applicant's summary and conclusion

Conclusions:

A 90% dechlorination under realistic application conditions was verified in 0.01 and 0.1 g/L aqueous solutions within 0.5 and 14 hours, respectively. At an initial concentration of 0.01 g/L, the substance was not detectable after 5 hours of exposure to "dirty" conditions. Chloramin B in higher concentrations 1-50g/L however was rather stable even under dirty conditions.

Executive summary:

In a non-GLP study, aqueous solutions (application solutions) of the test material at concentrations 0.01, 0.1, 1, 10, 20 and 50 g/L were exposed to „clean“ and „dirty“ conditions. „Clean” conditions: 1 L of the Chloramin B application solution, 0,3 g Albumin (Adult Bovine Serum, Lot B01110-2358); “Dirty” conditions: 1 L of Chloramin B application solution, 3 g Albumin (Adult Bovine Serum, Lot B01110-2358), 3 ml sheep erythrocytes. The “dirty” conditions were meant to simulate situations, were disinfection measures are required. The “dirty” conditions are equivalent to the conditions for efficacy testing of disinfectants. Samples were taken at regular intervals (hours and days) in order to measure the decomposition/dechlorination of the test material. The following analytical methods were applied: A validatedHPLC Method for the concentration 0.01 and 0.1 g/L and a validated iodometric titration methodfor the concentrations1 – 50 g/L. The measurements showed that at an initial concentration of 0.01 g/L, the substance was not detectable after 5 hours of exposure to "dirty" conditions.

A 90% dechlorination under realistic "dirty" application conditions was verified in 0.01 and 0.1 g/L aqueous solutions within 0.5 and 14 hours, respectively. Dechlorination in clean solutions was longer, i.e. 90% of 0.01 g/L solution were dechlorinated within 95 hours and abolut 50% of 0.1 g/l solutoin within 71 hours. Chloramin B in higher concentrations 1 -50g/L however was rather stable even under “dirty” conditions. Taking into consideration that during its way to the STP Chloramine B is diluted with the other waste waters - highly contaminated with bacteria and organic materials - and that a worst case dilutions factor of 500 (50 g/L time 0.1 g/L) is very realistic, it can be concluded that no Chloramine B is reaching the STP but its dechlorinated form – benzenesulphonamide.