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EC number: 225-805-6
CAS number: 5089-70-3
Combined Limit/Range-finding Test
The combined limit/range-finding test showed no inhibition of the
respiration rate at 10 and 100 mg/l and 29% at 1000 mg
(3-chloropropyl)triethoxysilane per litre. Therefore, the EC50 was
above the highest concentration tested (1000 mg/l).
There was no oxygen uptake from abiotic processes.
Final Dose Response Test
No statistically significant inhibition of the respiration rate of
the sludge was recorded up to 320 mg (3-chloropropyl)triethoxysilane per
litre. At 1000 mg/l, the inhibitory effect of the test item on aerobic
wastewater (activated sludge) bacteria showed 16% inhibition.
Table 3: Final Test Overview of Results
1) The amount of suspended solids in
the final test mixture was 1.5 g/l.
* Statistically significantly different compared to control.
Toxicity to microorganisms: ASRI
3-hour EC50 >1000 mg/l, NOEC 320 mg/l (loading rate) (OECD
An activated sludge
respiration inhibition 3-hour EC50 value of >1000 mg/l and a
NOEC value of 320 mg/l were determined for the registered substance,
(3-chloropropyl)triethoxysilane (CAS 5089-70-3, EC 225-805-6) in a
reliable study conducted according to an appropriate test protocol (OECD
209, adopted 22 July 2010), and in compliance with GLP (Charles River,
A supporting study reported an
activated sludge respiration inhibition 5-hour EC10 value of
2.0 ml/l (equivalent to 2.06 g/L based on a density of 1.03 g/cm3, Pseudomonas
putida) based on oxygen consumption/respiration inhibition was
determined for (3-chloropropyl)triethoxysilane (CAS 5089-70-3; EC No.
225-805-6) using a relevant test method and in compliance with GLP
(Schoberl 1994). The result is considered to be reliable but is not used
as key study because (i) the study is a 5-hour study instead of the
recommended 16 hour growth inhibition type test recommended in ISO 10712
Test Method, (ii) the study uses an emulsifier (nonylphenol ethoxylate)
to improve solubility of the test substance, and (iii) no reference
substance was used during the study.
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