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EC number: 236-675-5
CAS number: 13463-67-7
- Average TiO2 concentrations were: 238.3 ±
13.8, 230.7 ±
18.0, 182.5 ±
9.5 and 128.8 ±
12.5 µg/L after 1, 4, 8, and 24 h, respectively.
- Exposure concentration only verified in an additional laboratory
experiment at 250 µg TiO2/L at t=0 and t=24 h and not directly in the
mesocosms, hence exposure concentration within the mesocosms was not
Physicochemical water parameters:
- no significant differences in any of the measured water parameters
between tanks on Day 0
- no significant differences in following water parameters during the
experiment between treatments and controls (dissolved oxygen, water
temperature, salinity, conductivity, water tranparencey, pH, total
dissolved solids and total suspended soldis)
- total water volume per mesocosm was reduced by ~30% toward the end of
the experiment due to evaporation
- PAR light intensity increased only significantly by 5% on average
throughout the water column in the TiO2 250 µg/L treatment compared to
- TN, NH4 +-N, NO2 +NO3 and TP levels were unaffected by the treatments
- Dose-dependent reduction of SRP levels in TiO2 treatments compared to
the control; The concentration was reduced by 15 and 23% in the 25 and
250 µg/L TiO2 treatments compared to the control.
- No significant differences between controls and TiO2 treatments
observed for, Chl-a, carotenoids concentrations, biomass of Cladocera or
Copepoda, zooplankton grazing pressure on phytoplankton, macrophytes
PVI%, biomass of mactrophytes, biomass of periphyton, biomass and
abundance of Chironomids.
- two out of 72 fish died during the experiment, one each in the 250
µg/L TiO2 treatment group and in the controls; no visible signs of
lesions or infections on any deceased of living fish, no difference in
biomass between treatments and control.
- Significant reduction of the average biomass of all sampling points of
Rotifera at 25 µg/L by 32% and 250 µg/L by 57% compared to the controls.
- Rotifer biomass (µg/L) at test start (day 0) in the controls and 25
and 250 µg/L TiO2 treatment groups was 2.12 ±
0.32, 3.37 ±
1.63 and 1.79 ±
0.41 µg/L, and thus was not comparable within the different
mesocosms. Rotifer biomass in control and 25 µg/L TiO2 treatments
increased up to around 10 µg/L (graphical estimate) and 6.5 µg/L until
day 16 of exposure and declined to values below 2 µg/L at day 65. In
comparison, rotifer biomass in the 250 µg/L treatment groups remained
between 2 and 4 µg/L during the whole testing period.
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