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EC number: 236-675-5 | CAS number: 13463-67-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
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- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
Description of key information
Several studies are available and indicate that nano- and microsized TiO2 (including anatase, rutile and mixed forms) in natural soil and artificial substrate do not affect survival, reproduction and growth of Eisenia fetida and Eisenia andrei as well as survival and reproduction of Enchytraeus crypticus up to at least 1000 mg/kg.
Key value for chemical safety assessment
Additional information
Regarding toxicity to the reproduction of Eisenia fetida and Eisenia andrei, Schlich et al. (2012) and Wyrwoll et al. (2014) did not observe effects of nanosized and microsized TiO2 up to 1000 mg/kg dw soil:
In the study by Wyrwoll et al. (2014), dispersions of the
microsized TiO2 material Tiona AT1 (200 nm, anatase), and of the
nano-sized TiO2 materials Hombikat UV 100 (7-10 nm, anatase) and PC 105
(15-25 nm, anatase) in a natural test soil did not affect the survival
of Eisenia fetida in the acute limit toxicity test (OECD 207) and did
not affect growth and reproduction in the earthworm reproduction limit
test (OECD 222) resulting in 28-d NOEC values of ≥ 1000 mg/kg soil dw
(nominal) for survival and reproduction. Schlich et al. (2012) tested
three different nano-sized TiO2 materials, i.e. NM 105 (21 nm,
rutile/anatase), NM 101 (8 nm, anatase) and NM 103 (20 nm, rutile,
coated), in a standard toxicity test according to OECD 222 (2004). The
TiO2 materials did not reduce the number of offspring, and adults of
Eisenia andrei when applied to the test system via powder or dispersion
on feed or soil. Depending on the test setup, the 56-d and 28-d NOEC
values ranged from ≥ 20 (dispersion) to ≥ 1000 mg/kg soil dw (powder;
nominal). On the contrary, significant stimulation of reproduction was
observed for some TiO2 materials (P25, NM 101) with increasing levels.
However, the effect may be due to an improvement of the test soil.
Additionally, information is available on the toxicity of nanosized TiO2 to Enchytraeid worms from a study by Hund-Rinke et al. (2016): In a standard toxicity test according to OECD 220 (2004), the two different nano-sized TiO2 materials NM-104 (26 nm, rutile, Al-coated) and NM 105 (P25; 21 nm, anatase/rutile) did not affect the survival and reproduction of Enchytraeus crypticus (28 d EC10 and EC50 > 1000 mg TiO2 NP/kg soil dw (nominal)).
Several supporting studies on the toxicity of nanosized and microsized TiO2 to earthworm exist:
Micro-TiO2 material Titanium oxide-325 mesh (< 40 µm) did not affect survival and reproduction of Eisenia andrei and survival, reproduction and juvenile growth of Eisenia fetida in a sandy-loam soil resulting in 28-d, 56-d and 126 d NOEC values of ≥ 200 mg/kg dw (nominal) in a study by McShane et al. (2012). Higher concentrations were applied to an artificial substrate and the natural soil by dry-mixing resulting in 28-d and 56-d NOEC values of ≥ 10,000 mg/kg soil dw (nominal) for all endpoints. The toxicity of nanosized TiO2 material to the survival and reproduction of Eisenia andrei and Eisenia fetida was studied in artificial substrate and in a natural soil, the latter only with E. Andrei, according to OECD 207 and OECD 222, resulting in 28-d and 56-d NOEC values of ≥ 10,000 mg/kg soil dw (nominal) for adult survival and reproduction. However, tests were performed in the dark to avoid potential phototoxicity and thus potentially reducing the exposure of the worms. Under standard-light-darkness cycles, earthworms tend to remain in the soil under light conditions. Hence, results of these experiments may not be conservative and are considered supporting data.
Further, McShane et al. (2012) observed in an earthworm avoidance test with Eisenia fetida that the avoidance behaviour was not affected by microsized TiO2 at 10,000 mg/kg soil. Results for the three nano-sized TiO2 materials vary but indicate that earthworms do not avoid TiO2-NP concentrations up to 100 mg/kg soil but consistently avoid higher levels of 10,000 mg/kg soil.
Whitfield Aslund et al. (2012) used metabolomics to examine the response of Eisenia fetida earthworms raised from juveniles for 20−23 weeks in soil spiked with 20 or 200 mg/kg nano- and microsized TiO2. Data seem to indicate that earthworms exhibited changes in their metabolic profile relative to the control after exposure to 200 mg/kg of nano- and microsized TiO2. However, the correlation between the observed metabolomic response and ecologically relevant endpoints is missing to date.
Lapied, E. et al. (2011) exposed Lumbricus terrestris for 2-8 weeks to a TiO2 nanocomposite (TiO2 core coated with superposed layers of Al(OH)3 and PDMS) mixed into food or soil at concentrations up to 100 mg/kg. Apoptosis was measured by immunohistochemistry. Mortality and apoptotic frequency were not affected in soil-exposed worms. Based on Ti localization by XRF microscopy, nanoparticles appear to be rather inert and did not cross the intestinal epithelium/ chloragogenous matrix barrier to enter the coelomic liquid, or the cuticular barrier to reach the muscular layers.
The fact that direct exposure to TiO2 NPs did not impair the immuno-effectiveness of earthworm Eisenia veneta (Rosa) coelomocyte cells at concentrations well above those predicted for sewage sludge in the UK (25 mg/L) appears to be further evidence of the low potential of TiO2 nanoparticles for toxicity to earthworms (Johnson et al. 2011).
In sum and applying a weight of evidence approach, nano- and microsized TiO2 (rutile, anatase and mixed forms) appear to have a low potential for short- and long-term toxicity to soil annelids based on data available for different earthworm species and one enchytraeid species up to at least 1000 mg/kg dw soil.
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