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Toxicity to aquatic plants other than algae

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Dispersions of TiO2 nanoparticles did not affect the growth rate and yield of Lemna minor up to the highest tested concentration of 100 mg TiO2/L (nominal) and a 7 d EC50 > 100 mg TiO2/L as well as a 7 d NOEC ≥ 100 mg TiO2/L (nominal, growth rate and yield) were determined. Thus, the toxic potential of nanosized TiO2 to aquatic plants is low. A similar low potential can safely be assumed for microsized TiO2.

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Additional information

Nanosized TiO2:

In a 7-d GLP compliant test following OECD test guideline 221, dispersions of TiO2 nanoparticles (TiO2-uf-2, anatase, particle size: 19 nm) did not affect the growth rate and yield of Lemna minor up to the highest tested concentration of 100 mg TiO2 NP/L (nominal) and a 7 d EC50 > 100 mg TiO2/L as well as a 7 d NOEC ≥ 100 mg TiO2/L (nominal, growth rate and yield) were determined. Growth rate and yield were based on frond number, total frond area and biomass (dry weight).

Furthermore, one supporting study on the toxicity of nanosized TiO2 to Lemna minor is available: Dispersed nano-TiO2 , i.e. Aeroxide P25, particle size: 27.6 ± 11 nm, did not affect the growth rate of Lemna minor up to the highest tested concentration of 90 mg n-TiO2/L (nominal) under laboratory light conditions in a test performed according to ISO 20079. An unbounded 7 d NOEC value of ≥ 90 mg/L was determined. Since the test medium was not described, the respective results are considered supporting data.

 

Both studies indicate a low toxic potential of nanosized TiO2 to aquatic plants.