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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study was performed to an EPA guidline which is very similar to the relevant OECD guideline.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EPA OTS 797.1400 (Fish Acute Toxicity Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
purity: 99.72 % (glacial MAA). Lot 5-0523-88. received from Rohm and Hass Company. Light yellow liquid stored at room temperature

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Samples for analytical measurements were taken at 0 and 96 hrs. Comtrol and glacial MAA fortified samples were also determined at each sampling period.

Test solutions

Vehicle:
no
Details on test solutions:
A diluter stock solution (38 mg/ml) was prepared by dissolving 76 g of test substance in 2 liters of dilution water. The injector volume was adjusted
to deliver 5 ml of stock to 1.9 liters of dilution water.

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Lot 5588 from Mt Lassen Trout Farms in Red Bluff, CA.
Control fish had a mean weight of 1.0 g and a mean length of 41 mm at the end of the test. The biomass loading was 0.093 g/l/day. Fish were temperature acclimated in the absence of food for 72 hours before intiation of the test.

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
47 - 48 mg CaCO3/l
Test temperature:
11 - 12 degree C
pH:
5.3 - 7.8
Dissolved oxygen:
8.2 - 9.4 mg/l
Nominal and measured concentrations:
Nominal 6.5, 13, 25, 50 and 100 mg/L
Measured 6.2, 12, 23, 46, and 97 mg/L
Details on test conditions:
Twenty fish per concentration plus a dilution water control were used in a duplicate nominal dosing regimine. Temperature, dissolved oxygen and pH measured at all test concentrations at 0, 48 and 96 hours.

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
12 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
85 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
65 % mortality ocurred at 97 mg/L. Sublethal behavior responses were noted in the 23, 46 and 97 mg/L groups.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In a valid guideline study with Salmo gairdneri (new name: Oncorhynchus mykiss), the 96 hr LC50 of the test substance was 85 mg/L.
Executive summary:

In a valid guideline study with Salmo gairdneri (new name: Oncorhynchus mykiss), the 96 hr LC50 of the test substance was 85 mg/L.