cis-4-[3-(p-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine

Sediment toxicity

Administrative data

Endpoint:

sediment toxicity: long-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08.03.- 02.09.1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: method for a sediment toxicity test developed by a BBA/IVA working group

Version / remarks:

Barrett et al., 1994

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

cis-4-[3-(p-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine

EC Number:

266-719-9

EC Name:

cis-4-[3-(p-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine

Cas Number:

67564-91-4

Molecular formula:

C20H33NO

IUPAC Name:

(2R,6S)-4-[3-(4-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Chironomus riparius

Details on test organisms:

TEST ORGANISM
- Common name: harlequin fly
- Source: originally obtained from a laboratory culture of the University of Sheffield (UK), cultures maintained in-house

Study design

Study type:

laboratory study

Test type:

static

Water media type:

freshwater

Type of sediment:

artificial sediment

Limit test:

no

Test conditions

Hardness:

2.51 mmol/L

Test temperature:

19- 20 °C

pH:

8.2

Dissolved oxygen:

9.1 mg/L

Conductivity:

628 µS/cm

Nominal and measured concentrations:

0 (control), 0.005, 0.025, 0.125 mg a.s./L (nominal)

Details on test conditions:

TEST SYSTEM
- Test container (material, size): 2 1glass beakers (tall form, 11.7 cm diameter)
- Sediment volume: 2 cm high layer of the articial sediment (corresponding to 237 g sediment).
- Weight of wet sediment with and without pore water:
- Overlying water volume: 18 cm height of reconstituted water
- Depth of sediment and overlying water: 2 cm sediment plus 18 cm water column
- Aeration: yes
- Aeration frequency and intensity: gently aerated via Pasteurpipettes

EXPOSURE REGIME
- No. of organisms per container (treatment): 25
- No. of replicates per treatment group: 3
- No. of replicates per control / vehicle control: 3
- Feeding regime: regularly fed with fish food extract

OVERLYING WATER CHARACTERISTCS
- Type of water: Elendt medium acc. to OECD 219

CHARACTERIZATION OF ARTIFICIAL SEDIMENT
- Composition (if artificial substrate): 1 % CaCO3 (Merck 2069),
10% sphagnum moss peat (weakly-middle decomposed, "Compo Naturgarten Gärtnertorf“, DIN 11540—175),
20% Kaoline (kaolinite content > 30 %),
69%, quartz sand (0421/ISSO-trocken, Fa. Gebr. Willersinn
Particle size: 3 80 % 0.063 - 0.2 mm)
- Method of preparation (if artificial substrate): dry components are blended thoroughly under the addition of some water
- pH dry matter and/or whole sediment: 6.5
- Total organic carbon (%): 5%

OTHER TEST CONDITIONS
- Photoperiod (day:night-rhythm): 16:8 hours
- Light intensity: ca 400-550 lux (white uorescent tube)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Daily assessments of behaviour, mortality and emergence. The number, time and sex of emerged adults is recorded.

Results and discussion

Effect concentrations

Key result

Duration:

20 d

Dose descriptor:

NOEC

Effect conc.:

0.125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

development rate

Details on results:

Analytical measurements: Mean measured test concentrations of Fenpropimorph at test start were in the range of 57.3% - 78.3% of nominal. The lower recovery rate at test start was due to the low water solubility and the rapid dissipation to sediment of the test substance. At the end of the test - after 20 days - Fenpropimorph could not be detected in the overlaying water due to adsorption.

Biological results: Fenpropimorph caused no effects on the chironomids up to the highest concentration tested, 0.125 mg a.s./L. There was neither a significant reduction in the numbers of emerged adults nor in the development of the larvae.
The results are summarized below in table 1.

Any other information on results incl. tables

Table 1:  Effect of Fenpropimorph on Chironomus riparius
Concentration [mg a.s./L] nominal	Control	0.005	0.025	0.125
Concentration [mg a.s./L] measured	Control	0.0035	0.015	0.082
No. of emerged midges	73	71	73	72
Emergence of inserted larvae [%]	97.3	94.7	97.3	96.0
No. of emerged midges males / females	35/38	37/34	40/33	37/35
Mean emergence time [d]	15.3	15.6	15.5	15.4

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

For fenpropimorph the NOEC was determined to be 0.125 mg a.s./L (nominal).