cis-4-[3-(p-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06.03.2009 - 14.01.2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1300 (Daphnid Chronic Toxicity Test)

Version / remarks:

April 1996

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Version / remarks:

adopted Oct. 2008

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

cis-4-[3-(p-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine

EC Number:

266-719-9

EC Name:

cis-4-[3-(p-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine

Cas Number:

67564-91-4

Molecular formula:

C20H33NO

IUPAC Name:

(2R,6S)-4-[3-(4-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain/clone: Daphnia magna Straus
- Age at study initiation, range: > 2, < 24 hours
- Source: department DUU/OO (Dr. Elendt—Schneider) of BASF
- Age of parental stock: 34 days
- Feeding during test: Yes, regularly
- Food type: algae (Desmodesmus subspicatus)
- Frequency: at least 3 times a week

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Test temperature:

21.3 —22.3 °C

pH:

7.70 —8.20

Dissolved oxygen:

7.6 - 9.7 mg/L

Nominal and measured concentrations:

0 (control), 1.5, 3, 6, 12 and 24 µg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass vessels
- Material, size, headspace, fill volume: test volume: 50 mL
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): renewal every 2 - 3 days
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dilution water "M4" (Elendt medium)

OTHER TEST CONDITIONS
- Adjustment of pH: 7.7- 8.2
- Photoperiod (day: night—rhythm): 16:8 h,
- Light intensity: 125 - 430 lux,

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
After 21 days parent mortality, body length, dry weight and reproductive performance was assessed.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

4.5 µg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 2

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

2.2 µg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Details on results:

Analytical verification of the test item concentrations was conducted in all treatments at day 0, 2, 9, 12, 14, 16, 19 and 21. Recoveries at the different sampling days ranged from 101.1 to 53.9% of the nominal values (freshly prepared and old test solutions). Biological results were based on nominal and time weighted mean test item concentrations. The day of first brood ranged from 8 to 21 and the number of offspring varied between 0 and 139. At test end the body length of the adult daphnids ranged from 3.9 to 4.3 mm and the dry weight of the adult daphnids ranged from 0.537 to 0.907 mg. All validity criteria of the standard chronic test were fulfilled. Significantly lower reproduction compared to the controls was observed in the treatment 6 µg fenpropimorph /L and higher. Body dry weight and body length of the parent daphnids was significantly affected at the highest test concentration 24 µg fenpropimorph /L.

Reported statistics and error estimates:

Analysis of Variance (Dunnett’s test) for reproduction body length and dry
weight.

Applicant's summary and conclusion

Conclusions:

Overall NOEC = 3 (2.2) µg a.i./L nominal (time weighted mean);
LOEC = 6 (4.5) µg a.i./L nominal (time weighted mean)