cis-4-[3-(p-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine

Toxicity to terrestrial plants

Link to relevant study record(s)

Reference

Endpoint:

toxicity to terrestrial plants: long-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18.10.1999 - 04.07.2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)

Version / remarks:

draft version from 1999

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.4150 (Terrestrial Plant Toxicity Tier I (vegetative vigor))

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.4000 (Background - Nontarget Plant Testing)

GLP compliance:

no

Specific details on test material used for the study:

formulation BAS 421 12 F, batch no. 99-1, content of a.s.: 750 g/L Reg. No. 108 406 (nominal)

Species:

Zea mays

Plant group:

Monocotyledonae (monocots)

Species:

Avena sativa

Plant group:

Monocotyledonae (monocots)

Species:

Allium cepa

Plant group:

Monocotyledonae (monocots)

Species:

Brassica oleracea var. capitata

Plant group:

Dicotyledonae (dicots)

Species:

Pisum sativum

Plant group:

Dicotyledonae (dicots)

Species:

Daucus carota

Plant group:

Dicotyledonae (dicots)

Test type:

vegetative vigour test

Study type:

laboratory study

Substrate type:

other: Compost (steam sterilized)

Limit test:

yes

Total exposure duration:

14 d

Test temperature:

15 °C - 20 °C

pH:

6.7

Moisture:

humidity 40% - 70%

Details on test conditions:

TEST SYSTEM
- Testing facility: BASF AG, Agrarzentrum Limburgerhof, Limburgerhof, Germany Fed.Rep.
- Test container (type, material, size):
- Amount of soil:
- Method of seeding:
- No. of seeds per container: 3 to 5, depending on plant species
- No. of plants per treatment: 12 to 20, depending on plant species
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control:

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location:
- Pesticide use history at the collection site:
- Collection procedures:
- Sampling depth (cm):
- Soil: Compost (steam sterilized)

- Soil taxonomic classification: Loamy Sand
- Soil classification system:
- Composition (if artificial substrate):
- Organic carbon (%): 2.7
- pH: 6.7
- Maximum water holding capacity (in % dry weigth):
- CEC:
- Pretreatment of soil:
- Storage (condition, duration):

NUTRIENT MEDIUM (if used)
- Description:

GROWTH CONDITIONS
- Photoperiod: 16 h light : 8 h dark
- Light source: Radium HRJ-T Planta, 400 W
- Light intensity and quality: additional light when outdoor illumination was less than 4500 lux
- Day/night temperatures: 15 °C - 20 °C
- Relative humidity (%): 40% - 70%
- Wind velocity:
- Air exchange rate:
- Watering regime and schedules: According to need. First watering 24 h after application of the test substance.
- Water source/type:
- Volume applied: 400L/ha
- Interval of applications:
- Method of application: use of a laboratory spraying cabin constructed by Fa. Schmitt, Sondermaschinenbau, D-55435 Gau Algesheim, Germany.
- Any pest control method/fertilization (if used): According to need.

ACCLIMATION PERIOD:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Plant damage, shoot weight

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Nominal and measured concentrations:

Control, 0.75 L/ha BAS 421 12 F (equivalent to 562.5 g a.s./ha) and 2.25 L/ha (equivalent to 1687.5 g a.s./ha). The test item was applied in 400 L water/ha.

Species:

Allium cepa

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

3.62 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth

Remarks on result:

other: equals 2.25 L formulation/ha, 1687.5 g fenpropimorph/ha

Species:

Avena sativa

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

0.54 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Remarks on result:

other: equals 0.75 L formulation/ha, 562.5 g fenpropimorph/ha

Species:

Brassica oleracea var. capitata

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

0.54 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Remarks on result:

other: equals 0.75 L formulation/ha, 562.5 g fenpropimorph/ha

Species:

Daucus carota

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

0.54 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Remarks on result:

other: equals 0.75 L formulation/ha, 562.5 g fenpropimorph/ha

Species:

Pisum sativum

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

0.54 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Remarks on result:

other: equals 0.75 L formulation/ha, 562.5 g fenpropimorph/ha

Species:

Zea mays

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

0.54 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Remarks on result:

other: equals 0.75 L formulation/ha, 562.5 g fenpropimorph/ha

Details on results:

No plant damages were observed in onion. Slight damages were observed in carrot, pea and oat in the 2.25 L/ha test item rate. Damages in cabbage and corn were observed in both application rates. Plant weight was statistically significant reduced in corn in the 2.25 L/ha test item rate (Dunnett-test, α = 0.05).

Reported statistics and error estimates:

Descriptive statistics, ANOVA, followed by Dunnett test (α = 0.05).

Conversion path explanation:

 

Application rate:	562.5	g a.i./ha
Pot area (diameter)	8.4	cm
Pot area (radius)	4.2	cm
Pot surface	55.4176944	cm²	calculation circular area :  A = π/4 * d²
corresponds to	5.5418E-07	ha
Application rate * pot surface	0.00031172	g
corresponds to	0.31172453	mg
Amount of soil in the pot
Height	7	cm
Volume	387.923861	cm^3	calculation Volume : V = (π*r²) * h
Amount of soil	581.885791	g	Amount of soil = Volume * default soil bulk density (1.5 g/cm³)
corresponds to	0.58188579	kg
Test item concentration per pot		0.53571429	mg/kg

For the application rate of 2.25 L formulation/ ha (corresponding to 3796.9 g fenpropimorph/ha) a test item concentration per pot of 3.62 mg/kg was calculated.

 

Table 1: Effect of fenpropimorph formulation BAS 421 12 F on plant biomass and plant condition (visible damage) 14 DAA
Treatment [L/ha]	Carrot	Cabbage	Pea	Corn	Oat	Onion
Mean plant weight [% of control]
Control	100.0	100.0	100.0	100.0	100.0	100.0
0.75	96.2	95.3	104.9	95.9	102.9	101.5
2.25	84.0	86.1	97.8	72.4 *	97.1	96.5
Mean visible damage [% damage compared to control]
Control	0.0	0.0	0.0	0.0	0.0	0.0
0.75	0.0	5.0	0.0	5.0	0.0	0.0
2.25	16.3	25.0	21.3	36.3	6.3	0.0

* Statistically significant difference compared to the control (Dunnett-test, a = 0.05)

 

Validity criteria fulfilled:

yes

Conclusions:

Based on the results of this study conducted under worst case greenhouse conditions, it can be concluded, that applications of BAS 421 12 F up to 0.75 L/ha caused no damages to non-target terrestrial plants. At a rate of 2.25 L/ha BAS 421 12 F caused less than 30 % plant weight reduction.

Description of key information

Based on the results of this study conducted under worst case greenhouse conditions, it can be concluded, that applications of a fenpropimorph formulation (BAS 421 12 F) up to 0.75 L/ha (corresponding to 0.54 mg active substance/kg dw soil) caused no damages to non-target terrestrial plants. At a rate of 2.25 L formulation/ha caused less than 30 % plant weight reduction.

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for terrestrial plants:

0.54 mg/kg soil dw