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Diss Factsheets
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EC number: 200-745-3 | CAS number: 71-00-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Density
Administrative data
Link to relevant study record(s)
- Endpoint:
- density
- Remarks:
- density
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29.06 - 16.07.2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study is recently performed according to OECD and GLP. The study is well documented and conducted.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 109 (Density of Liquids and Solids)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method A.3 (Relative Density)
- Deviations:
- no
- GLP compliance:
- yes
- Type of method:
- pycnometer method
- Specific details on test material used for the study:
- Batch no.: P816P001
Expiry date: 03-01-2019
Storage: room temperature (20+/-5°C), away from light and humidity - Key result
- Type:
- density
- Density:
- 1.449 g/cm³
- Temp.:
- 20 °C
- Remarks on result:
- other: +/- 0.0003
- Conclusions:
- The density of L-isoleucine is 1.4490 g/cm3 at 20°C.
- Executive summary:
In the current study the density of L-Isoleucine was determined according to OECD test guideline 109. The study was GLP. Using the pycnometer method, the density of L-Isoleucine was determined to be 1.4490 ± 0.0003 g/cm3at 20.0 ± 0.4°C.
The standard deviation was 0.0021 g/cm3, showing a good correlation of the two determinations. Therefore, the study is considered valid.
Reference
Density ρ was calculated following the below equation:
ρtest = [(m4 - m1)/(m3 - m1) - (m5 - m4)] * ρverd
with: ρverd = density of replacement liquid in g/cm3
which can be calculated as follows:
ρverd= (m3 - m1)/(m2 -m1) * ρwater
with:
ρtest = density of solid test item in g/cm3
ρverd= density of replacement liquid in g/cm3
m1 = mass of empty pycnometer in g
m2 = mass of pycnometer with water in g
m3 = mass of pycnometer with replacement liquid in g
m4 = mass of pycnometer with solid test item in g
m5 = mass of pycnometer with replacement liquid and test item in g
The error was calculated following the below equation:
∆ρ = [(2 * ∆m * 100%) / (m4- m1)] + [(4 * ∆m * 100%) / (m3– m1) – (m5- m4)] + [2 * ∆m * 100%) / (m3- m1)] + [(2 * ∆m * 100%) / (m2- m1)]
with:
∆ρ = relative error of the measured density
∆m = absolute error of single weighing
Single determinations:
Determination |
Density (g/cm3) |
Error of determination (g/cm3) |
1 |
1.4475 |
0.0003 |
2 |
1.4294 |
0.0003 |
3 |
1.4505 |
0.0003 |
Note: the duplicate determination (exp 1 and 2) showed unusual variation. Therefore, a third determination was undertaken.
As the third determination confirmed the result of the first determination, experiments 1 and 3 were used for evaluation.
Description of key information
The density of L-isoleucine is 1.4490 g/cm3 at 20°C.
Key value for chemical safety assessment
- Relative density at 20C:
- 1.449
Additional information
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