Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 701-197-2 | CAS number: -
The results of the mouse lymphoma assay are shown in Table 1. The assays were first performed over a wide range of concentrations, then rerun over the narrow ranges shown in the table to demonstrate dose response. The relative growth column is an index of the amount of toxicity to the cells. In most cases the range of concentrations was restricted to 5-fold or less due to toxicity. The mutation frequency was calculated by dividing the number of colonies appearing in the selective plates (TFT) by the number of surviving cells as determined by plating a sample of cells in the absence of the selective agent. The mutation index was calculated by dividing the mutation frequency of the test results by the mutation frequency of the solvent control. For this particular set of experiments, we considered a compound positive if dose-related increases in the mutation index over at least 3 concentrations with the highest response at least equal to 3.0 were obtained. The mouse lymphoma assays were performed without metabolic activation, with metabolic activation by non-induced rat liver microsome preparation, and with an Aroclor-1254-induced rat liver microsome preparation.
In general, the highest mutagenic responses were obtained in the assays without metabolic activation. Responses obtained were slightly reduced in the assays that used the non-induced S9 preparations, whereas much lower responses were obtained with the Aroclor-induced S9 preparations. Cell toxicity was reduced proportionally, i.e. the same dose of chemical produced a more toxic response in the absence of any S9 and a less toxic response in the presence of the S9.
Table 1: Results of the L5178Y TK+/- mouse lymphoma mutagenicity assay for glycidol and Butyl glycidyl ester ± S9 mix
Relative growth (percent of control)
Mutation frequency per 10exp6 survivors
Mutation index (ratio of test/control)
Butyl glycidyl ester
a Without exogenous metabolic activation.
b With Aroclor-induced S9 fraction.
c With uninduced S9 fraction.
d Positive control without S9 fraction was ethyl methanesulfonate, 620 µg/ml; with induced S9 fraction, 2-acetylaminofluorene, 100 µg/ml; with uninduced S9 fraction, dimethylnitrosamine, 74 µg/ml.
e Positive control with S9 fraction for this compound only was benzo[a]pyrene, 3 µg/ml.
In a mammalian gene mutation assay (Mouse lymphoma assay, similar to OECD 476). L5178Y cell cultures were exposed to the read-across substances glycidol and butyl glycidyl ester for '1,2,3-propanetriol, glycidyl ethers' at concentrations of 8, 15, 23, 30, 45, 60, 75, 94, 125, 187, 250 µg/ml (glycidol) or 84, 100, 130, 164, 200, 256, 300, 320, 400, 500, 640, 800 µg/ml with and without metabolic activation. The metabolic activation system was either liver homogenates prepared from Aroclor-1254-induced Sprague-Dawley rats or rats injected with corn oil (non-induced).
Glycidol and butyl glycidyl ester were both tested up to cytotoxic concentrations, i.e. the highest dose was set at twice the level that killed 50% of the organisms in the toxicity assay.
Positive controls (ethyl methanesulfonate, 620 µg/ml, –S9; 2-acetylaminofluorene, 100 µg/ml, + induced S9; dimethylnitrosamine, 74 µg/ml, + uninduced S9) induced the appropriate responses. For both glycidol and butyl glycidyl ester there was a concentration-related positive response as well as the stipulated at least three-fold increase of the mutation frequency over background without or with both available metabolic activation systems. So it can be concluded that '1,2,3-propanetriol, glycidyl ethers' has to be considered as mutagenic in the mouse lymphoma assay, too.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again