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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well-documented publication, which meets basic scientific principles
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
publication
Title:
The Toxicology of Epoxy Resins
Author:
Hine, C.H., Kodama, J.K., Anderson, H.H., Simonson, D.W., Wellington, J.S.
Year:
1958
Bibliographic source:
AMA archive s of industrial health / American Medical Association, 17, p. 129-144

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The irritating effect of the test material was tested on normal rabbit eyes by dropping 0.1 mL of a 20% suspension in propylene glycol on the center of the cornea. Readings were made at 1, 24 and 48 hours, with the cornea unstained and then stained with a 2% solution of fluorescein sodium. Injury was scored according to the method of Draize.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): EPON 562

Test animals / tissue source

Species:
rabbit
Strain:
other: Albino
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 2.0 - 3.2 kg

Test system

Vehicle:
other: propylene glycol
Controls:
other: yes the other eye served as control
Amount / concentration applied:
0.1 mL
Duration of treatment / exposure:
not specified
Observation period (in vivo):
48 hours
Number of animals or in vitro replicates:
no details given
Details on study design:
Dropping 0.1 mL of a 20 % suspension in propylene glycol on the centre of the cornea

SCORING SYSTEM: according to Draize

TOOL USED TO ASSESS SCORE: fluorescein

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 1, 24 and 48 hours
Score:
82
Max. score:
120
Reversibility:
not specified
Remarks on result:
other: severely irritating

Applicant's summary and conclusion

Interpretation of results:
irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
This well-documented publication gives a detailed information about the experiments conducted and meets basic scientific principles. Therefore it is considered to be of the high quality (reliability Klimisch 2). The validity criteria of the test system are fulfilled. EPON 562 was severely irritating to rabbit eyes (score 82 of 120).
Executive summary:

The eye irritation potential of EPON 562 was investigated by Hine et al.(Hine, 1958). The irritating effect was tested on normal rabbit (male Albino) eyes by dropping 0.1 mL of a 20% suspension in propylene glycol on the center of the cornea. Readings were made at 1, 24, and 48 hours, with the corneas unstained and then stained with a 2% solution of fluorescein sodium. Injury was scored according to the method of Draize. EPON 562 was severely irritating to rabbit eyes (score 82 of maximum 120).