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Ecotoxicological information

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The toxicity of 1,2,3-Propanetriol, glycidyl ethers towards the aquatic biota was not investigated experimentally for all trophic levels. Guideline studies with GLP compliance were conducted regarding long-term toxicity to aquatic invertebrates (Liedtke, 2013), toxicity to algae (Liedtke, 2013) and to aquatic microorganisms (Wadsley, 2012). Other endpoints and supporting information are fulfilled using the scientifically acceptable QSAR software ECOSAR v0.99g and v1.00 (EPIWIN software by US-EPA) which is based on an extensive set of structure-activity relationships (SARs). The short-term results (ECOSAR v0.99g) are based on the SAR for "Diepoxides", whereas for long-term predictions (ECOSAR v1.00) the ECOSAR class “Epoxides, Poly” has been selected. For long-term toxicity, the Chronic Value is determined by the software tool. This value is defined as the geometric mean of the Lowest Observed Effect Concentration (LOEC) and the No Observed Effect Concentration (NOEC). Due to this, these values are not taken into account for the derivation of Predicted No Effect Concentrations (PNECs) for the aquatic compartment. For the main component with chlorine the following acute toxicity results were predicted for the three trophic levels: LC50(96h): 57.09 mg/L, EC50(48h): 4.38 mg/L and EC50(96h): 14324 mg/L for fish,Daphniaand green algae, respectively. The corresponding Chronic Values are: ChV(30d): 9.8 mg/L, ChV(21d): 209.8 mg/L and ChV(4d): 11676.6 mg/L. Concerning the main component without chlorine, the results differ only a little: LC50(96h): 55.17 mg/L for fish with a ChV(30d) of 10.2 mg/L, EC50(48h): 4.06 mg/L and ChV(21d): 226.2 mg/L for daphnids and EC50(96h): 33823 mg/L with a ChV(4d) of 12795.8 mg/L for algae. Additionally, two predictions have been run for acute toxicity to fish using read-across by the OECD QSAR Toolbox (v.3.3, 2014). EC50 of 72.4 and 79.3 mg/L for fish is predicted for GE-100 without and with chlorine component, respectively.

Liedtke (2013) investigated the long-term toxicity of the test substance towards Daphnia magna in accordance with OECD Guideline 211 / EU Method C.20. The semi-static test was conducted for 21 days with test medium renewals every 48 or 72 hours. The nominal test concentrations were chosen as: 1.0, 3.2, 10, 32 and 100 mg/L with 10 replicates per concentration. Based on the mentioned observations, it can be stated, with the exception of the reduced reproduction rates, no visible abnormalities were observed at the test animals during the experiment. The final results, based on survival and reproduction of the test animals can be reported as followed: NOEC(21d): 8.3 mg/L and LOEC(21d): 26 mg/L as mean measured concentrations. The EC50 value was clearly higher than the highest tested concentration of nominal 100 mg/L, because none of the responses exceeded 50 %.

Pseudokirchneriella subcapitata was used as test organism in an experiment conducted according to OECD Guideline 201 / EU Method C.3 (Liedtke, 2013). Nominal test concentrations of 0.32, 1.0, 3.2, 10, 32 and 100 mg/L were chosen for the definitive test. The biological results based on growth rate can be summarized as follows: ErC10(72h), ErC20(72h) as well as ErC50(72h) were found to be > 120 mg/L. Based on yield, the results were reported as: EbC10(72h): 7.7 mg/L, EbC20(72h): 120 mg/L (extrapolated) and EbC50(72h) > 120 mg/L (extrapolated). The NOEC was found as 21 mg/L and the LOEC as 69 mg/L after 72 hours exposure duration, respectively.

Concerning toxicity to aquatic microorganisms, an experiment was conducted in accordance to OECD Guideline 209 with activated sewage sludge, obtained from a predominantly domestic sewage (Wadsley, 2012). The following test concentrations were employed: test concentrations of 10, 100 and 1000 mg/L. Up to the highest concentration tested, no negative effects on the inoculum were observed. Therefore, the EC50(3h) is considered to be > 1000 mg/L, the corresponding NOEC is 1000 mg/L.