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EC number: 200-755-8 | CAS number: 71-48-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP, OECD guideline study (BASF) with acceptable restrictions (no E. coli or T102 tested, test substance not characterized)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- (no characterisation of the test substance, no E. coli or TA 102)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Cobalt dinitrate
- EC Number:
- 233-402-1
- EC Name:
- Cobalt dinitrate
- Cas Number:
- 10141-05-6
- IUPAC Name:
- cobalt(2+) dinitrate
- Details on test material:
- - Name of test material (as cited in study report): Co-Nitrat
- Physical state: fluid
- Analytical purity: not reported
- Lot/batch No.: 91/7671
- Stability under test conditions: not analyzed
- Storage condition of test material: room temperature
Constituent 1
Method
- Target gene:
- His operon
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- - Properly maintained: yes
- Additional strain / cell type characteristics:
- other: Defective excision repair system, reduced hydrophilic polysaccharide layer
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver S9 mix
- Test concentrations with justification for top dose:
- test substance: 20, 100, 500, 2500, 5000 µg/plate
positive controls
- 2-aminoanthracene: 2.5µg/plate
- 5-methyl-N'-nitro-N-nitrosoguanidine: 5 µg/plate
- 4-nitro-o-phenylendiamine: 10 µg/plate
- 9-aminoacridine chloride monohydrate: 100 µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: water (test substance); DMSO (positive controls)
- Justification for choice of solvent/vehicle: complete solubility
Controls
- Untreated negative controls:
- yes
- Remarks:
- Sterility controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Solvent controls
- Positive controls:
- yes
- Positive control substance:
- other: +S9: 2-aminoanthracene (TA 1535, 1537, 98, 100); -S9: 5-methyl-N'-nitro-N-nitrosoguanidine (TA 100, 1535), 4-nitro-o-phenylendiamine (TA 98), 9-aminoacridine chloride monohydrate (TA 1537)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation test
- Preincubation period: 20 minutes
- Exposure duration: 48 hours
METHOD OF APPLICATION: standard plate test
- Exposure duration: 48 hours
SELECTION AGENT (mutation assays): histidine
NUMBER OF REPLICATIONS: 2 experiments, á 3 culture/experiment
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth - Evaluation criteria:
- In general, a substance to be characterized as positive in the Ames test has to fulfil the following requirements:
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the results
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >= 2500 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- ADDITIONAL INFORMATION ON CYTOTOXICITY: occasionally observed >=2500 µg/plate depending on strain and test conditions
Any other information on results incl. tables
Maximum number of revertants (means ±SD):
In brackets: concentration of test substance
Standard Plate Test:
Strain | negative control | test substance | ||
-S9 | +S9 | -S9 | +S9 | |
TA 1535 | 18±1 | 24±3 | 16±2 (20 µg/plate) | 22±2 (20 µg/plate) |
TA 100 | 105±4 | 123±7 | 121±12 (2500 µg/plate) | 136±8 (100 µg/plate) |
TA 1537 | 9±2 | 10±2 | 8±1 (2500 µg/plate) | 10±3 (100 µg/plate) |
TA 98 | 25±3 | 46±2 | 25±3 (2500 µg/plate) | 48±5 (20 µg/plate) |
There was no increase in the number of his+ revertants in the standard plate test, neither in presence nor in absence of a metabolizing system.
Preincubation Test:
Strain | negative control | test substance | ||
-S9 | +S9 | -S9 | +S9 | |
TA1535 | 13±2 | 14±1 | 14±2 (20 µg/plate) | 13±1 (20 µg/plate) |
TA 100 | 103±3 | 102±7 | 103±7 (20 µg/plate) | 110±4 (500 µg/plate) |
TA 1537 | 7±2 | 8±2 | 6±2 (20 µg/plate) | 6±1 (100 µg/plate) |
TA 98 | 22±4 | 32±2 | 18±3 (500 µg/plate) | 32±2 (20 µg/plate) |
There was no increase in his+ revertants in the preincubation test, neither in presence nor in absence of a metabolizing system.
A slight decrease in the number of his+ revertants was occasionally observed depending on the strain and test conditions from about 2500 µg/plate onward.
The test substance was completely soluble in distilled water.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation
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