Registration Dossier

Administrative data

Endpoint:
toxicity to terrestrial plants: short-term
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
other information
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study, analytical dose verification.

Data source

Reference
Reference Type:
publication
Title:
Plant Toxicity Testing to Derive Ecological Soil Screening Levels for Cobalt and Nickel
Author:
Kapustka LA et al.
Year:
2006
Bibliographic source:
Environ Toxicol Chem 25(3): 865-874

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: E1963-98 (ASTM 1998)
GLP compliance:
not specified
Remarks:
presumably yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Analytical purity: no data

Sampling and analysis

Analytical monitoring:
yes

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
see below

Test organisms

open allclose all
Species:
other: Medicago sativa
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
- Common name: Alfalfa
- Plant family: Fabaceae
- Variety: Nitro Plus alfalfa variety
- Source of seed: purchased from Territorial Seed (Cottngs Grove, OR, USA; lot 18041).
- Prior seed treatment/sterilization: pretreated with a rhizobium inoculum by the supplier
- Historical germination of seed (germination of seed lot tested): initial germination percentage 87%
Species:
Hordeum vulgare
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
- Common name: Spring barley
- Plant family: Gramineae
- Source of seed: purchased from purchased from Wind River Seed (Manderson, WY, USA; lot 98-1544)
- Historical germination of seed (germination of seed lot tested): initial germination percentage was 100%
Species:
Brassica rapa
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
- Plant family: Brassicaeae
- Variety: Pac-Choy variety brassica
- Source of seed: purchased from Nichols Garden Nursery (Albany, OR, USA)
- Historical germination of seed (germination of seed lot tested): initial germination percentage was 91%
Species:
Raphanus sativus
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
- Common name: radish
- Plant family: Brassicaeae
- Variety: Early French Breakfast variety
- Source of seed: purchased from Nichols Garden Nursery
- Historical germination of seed (germination of seed lot tested): initial germination percentage was 77%
Species:
Lolium perenne
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
- Common name: ryegrass
- Plant family: Gramineae
- Variety: Penguin variety
- Source of seed: purchased from Seed Research (Corvallis, OR, USA; lot Y21-8-23)
- Historical germination of seed (germination of seed lot tested): Oregon State University Certified germination percentage after 13 d was 97.5%.

Study design

Test type:
seedling emergence toxicity test
Study type:
laboratory study
Substrate type:
other: artificial and natural soil
Total exposure duration:
14 d

Test conditions

Test temperature:
20.3-23.9°C
Moisture:
37.3-50.8%
Details on test conditions:
see below
Nominal and measured concentrations:
0,4.0,7.0, 12.3, 21.4, 37.5, 65.7, 114.9, 201.1, 351.9 mg/kg nominal
Reference substance (positive control):
yes
Remarks:
Boric acid, nominal concentrations of 160, 320, or 640 mg/kg for boron.

Results and discussion

Effect concentrationsopen allclose all
Species:
other: Medicago sativa
Duration:
14 d
Dose descriptor:
other: EC20
Effect conc.:
62.6 - 62.7 mg/kg soil dw
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Co
Basis for effect:
seedling emergence
Remarks on result:
other: for both soils
Species:
other: Medicago sativa
Duration:
14 d
Dose descriptor:
other: EC20
Effect conc.:
6.6 - 15.8 mg/kg soil dw
Nominal / measured:
meas. (not specified)
Conc. based on:
act. ingr.
Remarks:
Co
Basis for effect:
other: Geomean of shoot height, root length, shoot d.w., root d.w., total d.w.
Remarks on result:
other: for both soils
Species:
Hordeum vulgare
Duration:
14 d
Dose descriptor:
other: EC20
Effect conc.:
118 - 536.2 mg/kg soil dw
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Co
Basis for effect:
seedling emergence
Remarks on result:
other: for both soils
Species:
Hordeum vulgare
Duration:
14 d
Dose descriptor:
other: EC20
Effect conc.:
43.3 - 106.8 mg/kg soil dw
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Co
Basis for effect:
other: Geomean of shoot height, root length, shoot d.w., root d.w., total d.w.
Remarks on result:
other: for both soils
Species:
Raphanus sativus
Duration:
14 d
Dose descriptor:
other: EC20
Effect conc.:
393.2 - 496.2 mg/kg soil dw
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Co
Basis for effect:
seedling emergence
Remarks on result:
other: for both soils
Species:
Raphanus sativus
Duration:
14 d
Dose descriptor:
other: EC20
Effect conc.:
22.6 - 84 mg/kg soil dw
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Co
Basis for effect:
other: Geomean of shoot height, root length, shoot d.w., root d.w., total d.w.
Remarks on result:
other: for both soils
Results with reference substance (positive control):
- Results with reference substance valid?
Emergence and growth parameters for the negative and positive controls for each species were above the minimum acceptance standards for the tests.

Applicant's summary and conclusion