Cobalt di(acetate)

Toxicity to terrestrial plants

Administrative data

Endpoint:

toxicity to terrestrial plants: short-term

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

other information

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study, analytical dose verification.

Data source

Reference

Reference Type:

publication

Title:

Plant Toxicity Testing to Derive Ecological Soil Screening Levels for Cobalt and Nickel

Author:

Kapustka LA et al.

Year:

2006

Bibliographic source:

Environ Toxicol Chem 25(3): 865-874

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: E1963-98 (ASTM 1998)

GLP compliance:

not specified

Remarks:

presumably yes

Test material

Test material information

Constituent 1

Reference substance name:

Cobalt dichloride, hexahydrate

IUPAC Name:

Cobalt dichloride, hexahydrate

Constituent 2

Reference substance name:

7791-13-1

EC Number:

616-574-6

Cas Number:

7791-13-1

IUPAC Name:

7791-13-1

Details on test material:

- Analytical purity: no data

Sampling and analysis

Analytical monitoring:

yes

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

see below

Test organisms

1

Species:

other: Medicago sativa

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Alfalfa
- Plant family: Fabaceae
- Variety: Nitro Plus alfalfa variety
- Source of seed: purchased from Territorial Seed (Cottngs Grove, OR, USA; lot 18041).
- Prior seed treatment/sterilization: pretreated with a rhizobium inoculum by the supplier
- Historical germination of seed (germination of seed lot tested): initial germination percentage 87%

2

Species:

Hordeum vulgare

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: Spring barley
- Plant family: Gramineae
- Source of seed: purchased from purchased from Wind River Seed (Manderson, WY, USA; lot 98-1544)
- Historical germination of seed (germination of seed lot tested): initial germination percentage was 100%

3

Species:

Brassica rapa

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Plant family: Brassicaeae
- Variety: Pac-Choy variety brassica
- Source of seed: purchased from Nichols Garden Nursery (Albany, OR, USA)
- Historical germination of seed (germination of seed lot tested): initial germination percentage was 91%

4

Species:

Raphanus sativus

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: radish
- Plant family: Brassicaeae
- Variety: Early French Breakfast variety
- Source of seed: purchased from Nichols Garden Nursery
- Historical germination of seed (germination of seed lot tested): initial germination percentage was 77%

5

Species:

Lolium perenne

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: ryegrass
- Plant family: Gramineae
- Variety: Penguin variety
- Source of seed: purchased from Seed Research (Corvallis, OR, USA; lot Y21-8-23)
- Historical germination of seed (germination of seed lot tested): Oregon State University Certified germination percentage after 13 d was 97.5%.

Study design

Test type:

seedling emergence toxicity test

Study type:

laboratory study

Substrate type:

other: artificial and natural soil

Total exposure duration:

14 d

Test conditions

Test temperature:

20.3-23.9°C

Moisture:

37.3-50.8%

Details on test conditions:

see below

Nominal and measured concentrations:

0,4.0,7.0, 12.3, 21.4, 37.5, 65.7, 114.9, 201.1, 351.9 mg/kg nominal

Reference substance (positive control):

yes

Remarks:

Boric acid, nominal concentrations of 160, 320, or 640 mg/kg for boron.

Results and discussion

Effect concentrations

Effect concentrations 1

Species:

other: Medicago sativa

Duration:

14 d

Dose descriptor:

other: EC20

Effect conc.:

62.6 - 62.7 mg/kg soil dw

Nominal / measured:

meas. (not specified)

Conc. based on:

element

Remarks:

Co

Basis for effect:

seedling emergence

Remarks on result:

other: for both soils

Effect concentrations 2

Species:

other: Medicago sativa

Duration:

14 d

Dose descriptor:

other: EC20

Effect conc.:

6.6 - 15.8 mg/kg soil dw

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Remarks:

Co

Basis for effect:

other: Geomean of shoot height, root length, shoot d.w., root d.w., total d.w.

Remarks on result:

other: for both soils

Effect concentrations 3

Species:

Hordeum vulgare

Duration:

14 d

Dose descriptor:

other: EC20

Effect conc.:

118 - 536.2 mg/kg soil dw

Nominal / measured:

meas. (not specified)

Conc. based on:

element

Remarks:

Co

Basis for effect:

seedling emergence

Remarks on result:

other: for both soils

Effect concentrations 4

Species:

Hordeum vulgare

Duration:

14 d

Dose descriptor:

other: EC20

Effect conc.:

43.3 - 106.8 mg/kg soil dw

Nominal / measured:

meas. (not specified)

Conc. based on:

element

Remarks:

Co

Basis for effect:

other: Geomean of shoot height, root length, shoot d.w., root d.w., total d.w.

Remarks on result:

other: for both soils

Effect concentrations 5

Species:

Raphanus sativus

Duration:

14 d

Dose descriptor:

other: EC20

Effect conc.:

393.2 - 496.2 mg/kg soil dw

Nominal / measured:

meas. (not specified)

Conc. based on:

element

Remarks:

Co

Basis for effect:

seedling emergence

Remarks on result:

other: for both soils

Effect concentrations 6

Species:

Raphanus sativus

Duration:

14 d

Dose descriptor:

other: EC20

Effect conc.:

22.6 - 84 mg/kg soil dw

Nominal / measured:

meas. (not specified)

Conc. based on:

element

Remarks:

Co

Basis for effect:

other: Geomean of shoot height, root length, shoot d.w., root d.w., total d.w.

Remarks on result:

other: for both soils

Results with reference substance (positive control):

- Results with reference substance valid?
Emergence and growth parameters for the negative and positive controls for each species were above the minimum acceptance standards for the tests.