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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Publication which meets basic scientific principles with acceptable restrictions (e.g. no purity of the test substance are given).

Data source

Reference
Reference Type:
publication
Title:
Ergebnisse der Schadwirkung wassergefährdender Stoffe gegen Daphnia magna in einem weiterentwickelten standardisierten Testverfahren.
Author:
Bringmann G, Kühn R
Year:
1982
Bibliographic source:
Z Wasser Abwasser Forsch 15: 1-6

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
other: DIN 34812 part 11
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
The test follows the German National Standard Industrial Guideline DIN 38412 (part 11; draft), comparable to OECD 202.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
No further details on the test substance characteristics are given.

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Strain: IRCHA
Age: < 24 h
Supplier: not indicated

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h

Test conditions

Test temperature:
20 °C
pH:
8.0 ± 0.2
Details on test conditions:
- Stock solution:
2.59 g NaHCO3
0.23 g KCl
11.76 g CaCl2
4.93 g MgSO4
solved in 1 l deionized water. 25 ml of each solution is mixed and water is added to produce 1 l test water.
- Dissolved oxygen: aerated to saturation
- Exposure vessels: 50 ml glas beaker
- Exposure volume: 20 ml
- Exposure duration: 24 h
- Photoperiod: 15:9 hours day-night regime (E= 2.5 W/m²)
- Oxigen level after test: > 2 mg/l
- Number of replicates: 4
- Number of animals per replicate: 10

- Observations: immobilsation

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
6 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: neutralized
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
95 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: not neutralized
Reported statistics and error estimates:
Statistics: Litchfield-Wilcoxon probit analysis

Any other information on results incl. tables

EC values from neutralised tests:
EC0: 950 mg/L
EC50: 6000 mg/L
EC100: 7145 mg/L

EC values from non-neutralised tests:
EC0: 78 mg/L
EC50: 95 mg/L
EC100: 114 mg/L

Applicant's summary and conclusion