Cobalt di(acetate)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Publication which meets basic scientific principles with acceptable restrictions (e.g. no purity of the test substance are given).

Data source

Reference

Reference Type:

publication

Title:

Ergebnisse der Schadwirkung wassergefährdender Stoffe gegen Daphnia magna in einem weiterentwickelten standardisierten Testverfahren.

Author:

Bringmann G, Kühn R

Year:

1982

Bibliographic source:

Z Wasser Abwasser Forsch 15: 1-6

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: DIN 34812 part 11

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

The test follows the German National Standard Industrial Guideline DIN 38412 (part 11; draft), comparable to OECD 202.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Acetic acid

EC Number:

200-580-7

EC Name:

Acetic acid

Cas Number:

64-19-7

IUPAC Name:

acetic acid

Details on test material:

No further details on the test substance characteristics are given.

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Strain: IRCHA
Age: < 24 h
Supplier: not indicated

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test conditions

Test temperature:

20 °C

pH:

8.0 ± 0.2

Details on test conditions:

- Stock solution:
2.59 g NaHCO3
0.23 g KCl
11.76 g CaCl2
4.93 g MgSO4
solved in 1 l deionized water. 25 ml of each solution is mixed and water is added to produce 1 l test water.
- Dissolved oxygen: aerated to saturation
- Exposure vessels: 50 ml glas beaker
- Exposure volume: 20 ml
- Exposure duration: 24 h
- Photoperiod: 15:9 hours day-night regime (E= 2.5 W/m²)
- Oxigen level after test: > 2 mg/l
- Number of replicates: 4
- Number of animals per replicate: 10

- Observations: immobilsation

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

6 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks on result:

other: neutralized

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

95 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks on result:

other: not neutralized

Reported statistics and error estimates:

Statistics: Litchfield-Wilcoxon probit analysis

Any other information on results incl. tables

EC values from neutralised tests:
EC0:    950 mg/L
EC50:   6000 mg/L
EC100:  7145 mg/L

EC values from non-neutralised tests:
EC0:      78 mg/L
EC50:     95 mg/L
EC100:   114 mg/L