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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Publication which meets basic scientific principles with acceptable restrictions (e.g. no purity of the test substance are given).

Data source

Reference
Reference Type:
publication
Title:
Grenzwerte der Schadwirkung wassergefährdender Stoffe gegen Bakterien (Pseudomonas putida) und Grünalgen (Scenedesmus quadricauda) im Zellvermehrungshemmtest.
Author:
Bringmann G, Kühn R
Year:
1977
Bibliographic source:
Z Wasser Abwasser Forsch 3: 87-98

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Alga growth inhibition test according to Bringmann & Kühn
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- No further details on the test substance characteristics are given.

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Scenedesmus quadricauda

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
8 d

Test conditions

Test temperature:
27 °C
pH:
neutralized
Details on test conditions:
CULTURE CONDITIONS
- Culture vessels: 100 ml Erlenmeyer flasks
- Culture volume: 20 ml (nutrient solution 1)
- Light conditions: continous white light
- Temperature: 27 °C
- Pre-cultures: in sterilised Erlenmeyer flasks in 20 ml medium

TEST CONDITIONS
- Dilution water chemistry: sterile bidest. water
- Exposure vessels: 18 x 180 mm Kapsenberg culture vessels
- Exposure volume: 10 ml
- Number of replicates: 3
- Initial cell number: E(575 nm) = 20 for 10 mm layer thickness
- Exposure duration: 8 days
- Test temperature during the test: 27 °C
- pH values during the test: neutralised
- Photometric determination of absorbance at 578 nm after 8 days.

Results and discussion

Effect concentrations
Duration:
8 d
Dose descriptor:
other: TTC
Effect conc.:
4 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: neutralized, TTC (toxic threshold concentration) which is comparable with EC5

Applicant's summary and conclusion