Cobalt di(acetate)

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Publication which meets basic scientific principles with acceptable restrictions (e.g. no purity of the test substance are given).

Data source

Reference

Reference Type:

publication

Title:

Grenzwerte der Schadwirkung wassergefährdender Stoffe gegen Bakterien (Pseudomonas putida) und Grünalgen (Scenedesmus quadricauda) im Zellvermehrungshemmtest.

Author:

Bringmann G, Kühn R

Year:

1977

Bibliographic source:

Z Wasser Abwasser Forsch 3: 87-98

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Alga growth inhibition test according to Bringmann & Kühn

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Acetic acid

EC Number:

200-580-7

EC Name:

Acetic acid

Cas Number:

64-19-7

IUPAC Name:

acetic acid

Details on test material:

- No further details on the test substance characteristics are given.

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Scenedesmus quadricauda

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

8 d

Test conditions

Test temperature:

27 °C

pH:

neutralized

Details on test conditions:

CULTURE CONDITIONS
- Culture vessels: 100 ml Erlenmeyer flasks
- Culture volume: 20 ml (nutrient solution 1)
- Light conditions: continous white light
- Temperature: 27 °C
- Pre-cultures: in sterilised Erlenmeyer flasks in 20 ml medium

TEST CONDITIONS
- Dilution water chemistry: sterile bidest. water
- Exposure vessels: 18 x 180 mm Kapsenberg culture vessels
- Exposure volume: 10 ml
- Number of replicates: 3
- Initial cell number: E(575 nm) = 20 for 10 mm layer thickness
- Exposure duration: 8 days
- Test temperature during the test: 27 °C
- pH values during the test: neutralised
- Photometric determination of absorbance at 578 nm after 8 days.

Results and discussion

Effect concentrations

Duration:

8 d

Dose descriptor:

other: TTC

Effect conc.:

4 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: neutralized, TTC (toxic threshold concentration) which is comparable with EC5