Registration Dossier

Administrative data

Endpoint:
neurotoxicity
Remarks:
acute
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Abstract. Original reference not available.

Data source

Reference
Reference Type:
publication
Title:
Histofluorescence studies of monoamine neurons in the rat brain after cobaltous acetate intoxication.
Author:
Smialowska M, Bugera-Piekuch T, Bal A, Smialek M
Year:
1987
Bibliographic source:
Med Biol 65(4): 193-198

Materials and methods

Principles of method if other than guideline:
The aim was to study changes in brain monoamine neurons in an experimental animal model with an extrapyramidal motor syndrome of the parkinsonian type.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): cobaltous acetate
- Analytical purity: not reported
- Other: No information if anhydrate or tetrahydrate was used

Test animals

Species:
rat
Strain:
not specified
Sex:
not specified

Administration / exposure

Route of administration:
other: no data
Vehicle:
not specified

Examinations

Sacrifice and (histo)pathology:
- Type of staining: histofluorescence and histological staining

Results and discussion

Any other information on results incl. tables

Neurological signs were observed in rats after acute cobaltous acetate intoxication under mild ischemic conditions. Histofluorescence studies showed a decrease in catecholamine fluorescence (which signifies a decrease in the amine content) in the hypothalamus and mesencephalic reticular formation, but not in the substantia nigra or basal ganglia. Serotonin fluorescence was increased in nerve cell bodies of the dorsal and median raphe nuclei and in nerve terminals in some thalamic and preoptic regions. Histological staining of sections adjacent to the fluorescent ones showed no neuronal loss and some pathology of myelin.

Applicant's summary and conclusion