Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: inhalation

Currently viewing:

Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report date:
1980

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Principles of method if other than guideline:
The test animals (10 female, 10 male rats) were exposed to atmospheres, saturated with test substance vapours for 4 hours.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Acrylic acid
EC Number:
201-177-9
EC Name:
Acrylic acid
Cas Number:
79-10-7
Molecular formula:
C3H4O2
IUPAC Name:
prop-2-enoic acid
Specific details on test material used for the study:
- Name of test material (as cited in study report): Acrylic acid, pure
- Analytical purity: > 99%

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Firma MUS RATTUS, Brunnthal.
- Weight at study initiation: 185 ± 15 g
- Housing: air-conditioned chambers
- Diet (ad libitum): Herilan MRH (Firma H. EGGERSMANN KG, Rinteln/Wesser)
- Water (ad libitum): tap water

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2
- Humidity (%): 55 ± 5
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
air
Details on inhalation exposure:
- Exposure apparatus:
The exposure apparatus consisted of whole-body inhalation system. Groups of 5 animals was restrained in cages and the cages were placed into a
stainless steel and glass exposure chamber with a capacity of 200 litres.

- System of generating vapours:
The test substance was pumped into an evaporator, whose temperature was maintened at 50°C, using a continuous infusion pump (UNITA I, B. Braun). The resulting vapours were mixed with the fresh air flow and conducted into the inhalation system.

- Brief description of analytical method used:
To monitor the test substance vapour concentration in the cages, an HP Model 5840 A gas chromatograph (GC) equipped with a flame
ionization detector was used.



Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
4.25 and 5.12 mg/L (analytically determined)
No. of animals per sex per dose:
10
Control animals:
yes
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: prior to exposure and on postexposure days 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, histopathology
Statistics:
The statistical assessment was based on the bionomial test (Wittig H.: mathematische Statistik, 1974, 32-35) performed by BASF data processing center.

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 5.1 mg/L air (analytical)
Exp. duration:
4 h
Mortality:
No rats died during exposure or during the 14-day postexposure period.
Clinical signs:
other: Clinical signs observed on the day of exposure included reddish periocular and perinasal lacrimination, salivation, eyelid closure, scrubby clotted skin and dyspnoea. The animals of both groups were 4 and 5 days respectively after the exposure without
Body weight:
A lower body weight gain was observed (both sexes) on both postexposure Days 7 and 14 relative to the control group.
Gross pathology:
No macroscopic lesions were observed in animals sacrificed at the end of the 2-week postexposure period.

Any other information on results incl. tables

Table 1: Results

Group

Dose

mg/L

Mortality

Dead/Treated

male

female

2

4.25

0/10

0/10

1

5.12

0/10

0/10

Applicant's summary and conclusion

Interpretation of results:
Category 3 based on GHS criteria