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Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report date:
1980

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Principles of method if other than guideline:
The test animals (10 female, 10 male rats) were exposed to atmospheres, saturated with test substance vapours for 4 hours.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Acrylic acid
EC Number:
201-177-9
EC Name:
Acrylic acid
Cas Number:
79-10-7
Molecular formula:
C3H4O2
IUPAC Name:
prop-2-enoic acid
Specific details on test material used for the study:
- Name of test material (as cited in study report): Acrylic acid, pure
- Analytical purity: > 99%

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Firma MUS RATTUS, Brunnthal.
- Weight at study initiation: 185 ± 15 g
- Housing: air-conditioned chambers
- Diet (ad libitum): Herilan MRH (Firma H. EGGERSMANN KG, Rinteln/Wesser)
- Water (ad libitum): tap water

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2
- Humidity (%): 55 ± 5
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
air
Details on inhalation exposure:
- Exposure apparatus:
The exposure apparatus consisted of whole-body inhalation system. Groups of 5 animals was restrained in cages and the cages were placed into a
stainless steel and glass exposure chamber with a capacity of 200 litres.

- System of generating vapours:
The test substance was pumped into an evaporator, whose temperature was maintened at 50°C, using a continuous infusion pump (UNITA I, B. Braun). The resulting vapours were mixed with the fresh air flow and conducted into the inhalation system.

- Brief description of analytical method used:
To monitor the test substance vapour concentration in the cages, an HP Model 5840 A gas chromatograph (GC) equipped with a flame
ionization detector was used.



Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
4.25 and 5.12 mg/L (analytically determined)
No. of animals per sex per dose:
10
Control animals:
yes
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: prior to exposure and on postexposure days 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, histopathology
Statistics:
The statistical assessment was based on the bionomial test (Wittig H.: mathematische Statistik, 1974, 32-35) performed by BASF data processing center.

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 5.1 mg/L air (analytical)
Exp. duration:
4 h
Mortality:
No rats died during exposure or during the 14-day postexposure period.
Clinical signs:
other: Clinical signs observed on the day of exposure included reddish periocular and perinasal lacrimination, salivation, eyelid closure, scrubby clotted skin and dyspnoea. The animals of both groups were 4 and 5 days respectively after the exposure without
Body weight:
A lower body weight gain was observed (both sexes) on both postexposure Days 7 and 14 relative to the control group.
Gross pathology:
No macroscopic lesions were observed in animals sacrificed at the end of the 2-week postexposure period.

Any other information on results incl. tables

Table 1: Results

Group

Dose

mg/L

Mortality

Dead/Treated

male

female

2

4.25

0/10

0/10

1

5.12

0/10

0/10

Applicant's summary and conclusion

Interpretation of results:
Category 3 based on GHS criteria