Acrylic acid

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Principles of method if other than guideline:

The test animals (10 female, 10 male rats) were exposed to atmospheres, saturated with test substance vapours for 4 hours.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Acrylic acid

EC Number:

201-177-9

EC Name:

Acrylic acid

Cas Number:

79-10-7

Molecular formula:

C3H4O2

IUPAC Name:

prop-2-enoic acid

Specific details on test material used for the study:

- Name of test material (as cited in study report): Acrylic acid, pure
- Analytical purity: > 99%

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Firma MUS RATTUS, Brunnthal.
- Weight at study initiation: 185 ± 15 g
- Housing: air-conditioned chambers
- Diet (ad libitum): Herilan MRH (Firma H. EGGERSMANN KG, Rinteln/Wesser)
- Water (ad libitum): tap water

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2
- Humidity (%): 55 ± 5
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

- Exposure apparatus:
The exposure apparatus consisted of whole-body inhalation system. Groups of 5 animals was restrained in cages and the cages were placed into a
stainless steel and glass exposure chamber with a capacity of 200 litres.

- System of generating vapours:
The test substance was pumped into an evaporator, whose temperature was maintened at 50°C, using a continuous infusion pump (UNITA I, B. Braun). The resulting vapours were mixed with the fresh air flow and conducted into the inhalation system.

- Brief description of analytical method used:
To monitor the test substance vapour concentration in the cages, an HP Model 5840 A gas chromatograph (GC) equipped with a flame
ionization detector was used.

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

4.25 and 5.12 mg/L (analytically determined)

No. of animals per sex per dose:

10

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: prior to exposure and on postexposure days 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, histopathology

Statistics:

The statistical assessment was based on the bionomial test (Wittig H.: mathematische Statistik, 1974, 32-35) performed by BASF data processing center.

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 5.1 mg/L air (analytical)

Exp. duration:

4 h

Mortality:

No rats died during exposure or during the 14-day postexposure period.

Clinical signs:

other: Clinical signs observed on the day of exposure included reddish periocular and perinasal lacrimination, salivation, eyelid closure, scrubby clotted skin and dyspnoea. The animals of both groups were 4 and 5 days respectively after the exposure without

Body weight:

A lower body weight gain was observed (both sexes) on both postexposure Days 7 and 14 relative to the control group.

Gross pathology:

No macroscopic lesions were observed in animals sacrificed at the end of the 2-week postexposure period.

Any other information on results incl. tables

Table 1: Results

Group	Dose mg/L	Mortality Dead/Treated
		male	female
2	4.25	0/10	0/10
1	5.12	0/10	0/10

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria