Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

Acrylic acid is acutely harmful for freshwater and marine fish.

LC50 (96 h, flow through) = 27 mg/L (measured) (Salmo gairdneri, EPA OTS 797.1400)

LC50 (96 h, semi-static) = 222 mg/L (nominal) (Brachydanio rerio, 92/69/EEC part C1)

LC50 (96 h, flow through) = 236 mg/L (measured) (Cyprinodon variegatus, OECD TG 203)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
27 mg/L

Marine water fish

Marine water fish
Effect concentration:
236 mg/L

Additional information

Acrylic acid was tested in an acute toxicity test with Salmo gairdneri under flow-through conditions. Biological and chemical procedures followed the U.S. EPA-TSCA, 40 CFR, Part 797 Guideline 797.1400. Based on mean measured concentrations, the 96-hour LC50 was determined to be 27 mg/L. Reported behavioural effects were: loss of equilibrium, on bottom orientation, quiescence and erratic swimming (BAMM, 1990).

As supporting study, an acute fish test according to the 92/69/EEC part C1 with Brachydanio rerio using a semi-static test design is available. Analytical concentration control by spectrophotometrical measurements revealed a recovery rate of > 80 %. Therefore, effect values were based on nominal concentrations. The 96-hour LC50 was determined to be approx. 222 mg/L (Huels, 1995).


In a saltwater study conducted in the sheepshead minnow (BAMM, 1995), the 96-hour LC50 was estimated to be 236 mg/L. This study was conducted according to OECD Guideline 203 using a flow-through design. The LC50 value was based on analytically determined concentrations. Analytical recovery was between 90 and 110 % of nominal. Mortality occurred only in the highest concentration of 297 mg/L. Clinical signs and symptoms observed were: lying on bottom, lethargy, surfacing, loss of equilibrium. The NOEC was determined to be 187 mg/L. Even though being a OECD-TG-study conducted in compliance with GLP regulations, this study is only valid with restrictions since during a seven-hour-period a malfunction in the diluter occurred that caused test substance concentrations to rise up to 200 % of nominal.


Taking all these data into consideration, LC50 values for freshwater and marine fish are between 27 and 236 mg/L for acrylic acid. Salmo gairdneri appears to be the most sensitive fish species.

Several secondary literature sources reported short-term LD50 values with different fish species. Some species were not sensitive to the test item at other while other showed some effects.