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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1958
Report date:
1958

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
BASF-Test: Before OECD Guideline 405 was established, eye irritation was tested using an internal method (BASF test). White Vienna rabbits were used. One drop of the test substance was applied to the conjunctival sac of the right eye of the animal. The adjacent eye was treated with one drop of physiological solution of sodium chloride and served as a control.The animals were observed several times on the treatment day and 8 days afterwards, and findings were recorded on working days.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Acrylic acid
EC Number:
201-177-9
EC Name:
Acrylic acid
Cas Number:
79-10-7
Molecular formula:
C3H4O2
IUPAC Name:
acrylic acid
Specific details on test material used for the study:
- Name of test material (as cited in study report): Acrylic acid, raw, stabilised with approx. 0.2% phenothiazon.
- Analytical purity: 86%
- Composition of test material, percentage of components: Acrylic acid 86%, 2-propenoic acid, 2-carboxylethyl ester, 11%, water 2%,
phenothiazon 0.2%, unknown components 0.8%.

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: The adjacent eye was treated with one drop of physiological solution of sodium chloride and served as a control.
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied: approx. 50 µL


Duration of treatment / exposure:
21 days
Observation period (in vivo):
21 days
Number of animals or in vitro replicates:
2
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing: no

SCORING SYSTEM: The original BASF grading was converted into the numerical grading according to the OECD Draize system.

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Time point:
other: immediately after treatment
Score:
> 4
Reversibility:
not reversible
Remarks on result:
other: Observation of eyes revealed inmmediate corrosion after treatment
Irritant / corrosive response data:
One drop of indiluted Acrylic acid caused a severe corrosion of the conjunctiva and cornea as a result of an exposure period of 10 minutes. In the course of 8 to 14 days, a complete destruction of the eye was observed and the animal was sacrificed.

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye) based on GHS criteria