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According to a OECD test n°222, taking into account the survival, growth and reproduction rates of the exposed adult test organisms, the highest concentration of Acetalization product between glucose and C20/22(even numbered)-alcohol without toxic effects on Eisenia fetida (NOEC) after the test period was determined to be at least 1000 mg/kg dry soil. The NOEC might even be higher, but concentrations in excess of 1000 mg/kg dry soil were not tested.

The substances in the category are highly insoluble. For uptake by plants and microorganisms, the substance needs to go into solution (interstitial or pore water) before exposure can occur, therefore testing the substances in plants or microorganisms is considered irrelevant.