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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2006-11-12 tà 2007-02-15
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Physical state: solid, pearls of wax
- Analytical purity: dry extract >99%
- Composition of test material, percentage of components: arachidyl alcohol 50-60%, behenyl alcohol 25-35%, behenyl glucoside 10-20%
- Lot/batch No.: T62435
- Expiration date of the lot/batch: 14 june 2008 (re-test)
- Storage condition of test material: at the room temperature
- water solubility <0.1 g/L
- stability : water 96h

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
no specific analytic method available

Test solutions

Vehicle:
no
Details on test solutions:
As the solubility lies below 100 mg/L, the water-accommodated fraction was prepared for the test. This was done by weighing the nominal load, adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was filtrated through paper filters.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Variety: Straus
Strain: Berlin
Sex: female
Age: between 0 and 24 hours
Origin: Umweltbundesamt Berlin
The animals were kept for the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identical.
Vessels: glass beakers, nominal 2 L
Medium: M4-Medium
Medium renewal: twice a week
Photo period: 16/8 hours, using neon tubes
Temperature: 20 +/- 2 °C

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
2.50 mmol/L
Test temperature:
21.5 - 22.4°C
pH:
7.3 to 7.7
Dissolved oxygen:
8.5 mg/L
Nominal and measured concentrations:
100 mg/L
Details on test conditions:
TEST SYSTEM ALGUES
- Test vessel: glass beakers , nomminal volume 50ml, tall Shape, diameter 8mm
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel:five
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:deionised water with an enrichment of certain minerals

OTHER TEST CONDITIONS
- Adjustment of pH: 7.8-+0.2
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
ca. 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
ca. 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
validatation of the study:
- the 24h-EC50i of potassium dichromate = 1.4 mg/L
- immobilisation in the controls was 10% at the most
- the lowest concentration of dissolved oxygen at the end of the test was 8.6 mg/L
- the highest variation of pH-value was 0.2 unit.
Results with reference substance (positive control):
24h-EC50i of potassium dichromate = 1.4 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The following results for the test substance were obtained:

- 48h EC50i >= 100 mg/L
Executive summary:

This study was performed in order to evaluate the toxic potential of the substance towards freshwater shrimp,using the species Daphnia magna. Daphnia magna Straus, which belongs to the family of crustacea, was chosen in accordance to the OECD 202.

As the test item is poorly water soluble, the 'water-accommodated fraction' was used for the test. This was done by weighting the nominal load, adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was filtrated through 0.45 µm filters.

In the main study, 3 concentrations of the test item in a geometric series (spaces by factor 10, the highest being 100 mg/L) were used. 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.

No significant immobilisation was observed in the treatments and in the control.

The 24hEC50i of potassium dichromate was tested in a current reference test. The value was determined as 1.4 mg/L, lying within the demanded range of 0.6 -2.1 mg/L.

The following results for the test substance were obtained: - 24h NOEC = 100 mg/L - 48h NOEC = 100 mg/L - 24h EC50i >= 100 mg/L - 48h EC50i >= 100 mg/L