Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 216-245-3
CAS number: 1533-45-5
Individual Animal - Evaluation of
Test System : Sprague Dawley Rat
Sex : Male
Group : I
Dose : 2000 mg/kg body weight
D A Y S
Sex : Female
Table No. I
Summary of Clinical Signs of
Toxicity and Mortality
Sex : Male
Total Number of
Period of signs in days
From - to
No clinical signs observed
1 - 5
Day 0 - Day 14
Sex : Female
6 - 10
Table No. II
Summary of Evaluation of Dermal
Sex : Male
Period of signs
No dermal reaction observed
Mean Body Weight and Percent Body
Weight Gain (g)
(mg/kg body weight)
Body weight Day 0
Body weight Day 7
% body weight gain
Body weight Day 14
day 7- 14
day 0- 14
Summary of Gross Pathological
Gross Pathological Findings
No abnormality detected
TS = Terminal Sacrifice
A study was designed and conducted to
determine the dermal reaction profile of the test chemical in Sprague
Dawley rats. The study was performed as per OECD Guidelines 402 and
complying to the GLP procedures. Ten rats (5 male and 5 female) were
used for conducting dermal irritation /corrosion study.
The animals were kept in their cages
for at least 5 days prior to administration for acclimatization to the
laboratory condition and after acclimatization period, animals were
randomly selected. Approximately 24 hours before application, the hair
of each rat was closely clipped from the trunk (dorsal surface and sides
from scapular to pelvic area) with an electric clipper, so as to expose
at least 10% of the body surface area. The test item was moistened with
distilled water. The test item was applied onto the exposed skin of the
animal, taking care to spread the test item evenly over the entire area
of approximately 10% of the total body surface area or as much of the
area as can reasonably be covered. The test item was held in contact
with the skin using a porous gauze dressing and non irritating tape
around the animal to cover the exposure site for first 24 hours exposure
period. Elizabethan collar was placed on each animal for first 24 hours
after application of the test item. These collars prevent ingestion of
test item. Following 24 hours of exposure, the wrapping was removed and
the test site wiped free of excess test item. Distilled water was used
to remove residual test item.
The test item was applied to shorn
skin of 5 male and 5 female animals at 2000 mg/kg body weight.
Administration of the test item at 2000 mg/kg did not result in any skin
reaction at the site of application during the study period of 14 days.
Administration of the test item did not result in any signs of toxicity
and mortality during the study period of 14 days.
The overall irritation score of the
substance was determined to be 0 and no erythema and edema (skin
irritation) were found at the end of 14 days observation period after
Hence, it was concluded that the test
chemical was Non-Irritating to the skin of Sprague Dawley rats under the
experimental conditions tested and classified as “Category- Not
Classified” as per CLP Classification.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Deze website maakt gebruik van cookies om het surfen zo aangenaam mogelijk te maken.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again