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EC number: 216-245-3
CAS number: 1533-45-5
D.O Values (mg/L)
No. of Days
BOD Values (mg O2/mg)
3: PERCENT BIODEGRDATION RESULTS
BOD42, THOD AND % BIODEGRADATION VALUES
42-days Closed Bottle test following
the OECD guideline 301 D to determine the ready biodegradability of the
test chemical. The study was performed at a temperature of 20°C. The
test system included control, test item and reference item. Polyseed
were used for this study. 1 polyseed capsule were added in 500 ml D.I
water and then stirred for 1 hour for proper mixing and functioning of
inoculum. This gave the bacterial count as 107 to 108 CFU/ml. At the
regular interval microbial plating was also performed on agar to confirm
the vitality and CFU count of microorganism. The concentration of test
and reference item (Sodium Benzoate) chosen for both the study was 4
mg/L, while that of inoculum was 32 ml/l. OECD mineral medium was used
for the study. ThOD (Theoretical oxygen demand) of test and reference
item was determined by calculation. % degradation was calculated using
the values of BOD and ThOD for test item and reference item. The %
degradation of procedure control (reference item) was also calculated
using BOD & ThOD and was determined to be 75.3%. Degradation of Sodium
Benzoate exceeds 46.38% on 7 days & 61.44% on 14th day. The activity of
the inoculum was thus verified and the test can be considered as valid.
The BOD42 value of test chemical was observed to be 0.62 mgO2/mg. ThOD
was calculated as 2.31 mgO2/mg. Accordingly, the % degradation of the
test item after 42 days of incubation at 20 ± 1°C according to Closed
Bottle test was determined to be 26.83%. Based on the results, the test
item, under the test conditions, was considered to be primary inherently
biodegradable in nature.
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