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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
17-05-2018 to 19-05-2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
Acute Immobilization study of test material on Daphnia magna in a Semi-Static System for 48 hrs. Test performed according to the OECD guideline 202.
GLP compliance:
not specified
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
The test substance was partially soluble in water. Therefore, the stock solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring.After stirring the stock solution was analytically detected and the stock concentration was found to be 0.6450 mg/L. Limit test was set up with this concentration only.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching
Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
48 h
Post exposure observation period:
24 and 48 hrs
Hardness:
150.5 mg of CaCO3
Test temperature:
20±2 degree C
pH:
7.4
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 0.645 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Other details not known

9. OBSERVATIONS:

 

 

 

 

 

 

9.1. Immobility

 

 

 

 

 

 

Nominal

 

Number

of

Immobility

 

 

Concentration

Daphnids

0 h

24 h

 

48 h

(mg/l)

 

 

 

 

 

 

 

Control

 

10

-

-

 

 

-

0.6450

 

10

-

-

 

 

-

0.6450

 

10

-

-

 

 

-

Any

Abnormal

 

 

-

No observed effects.

No observed effects.

Behaviour (if any)

 

 

 

 

 

 

9.2. pH and oxygen content:

 

 

 

 

 

 

 

Nominal

 

Number of

pH(mg/L)

 

 

 

Concentration

daphnids

0h

48h

 

 

 

(mg/l)

 

 

 

 

 

 

 

Control

 

10

7.1

7.2

7.2

 

 

0.6450

 

10

7.3

7.4

7.4

 

 

0.6450

 

10

7.3

7.4

7.4

 

 

Nominal

 

Number of

DO(mg/L)

 

 

 

Concentration

daphnids

0h

48h

 

 

 

(mg/l)

 

 

 

 

 

 

 

Control

 

10

6.9

6.9

6.9

 

 

0.6450

 

10

6.8

6.8

6.8

9.3. Test temperature

0.6450

 

10

6.8

6.8

6.8

Nominal

 

Number of

Temparature

 

 

 

 

 

 

 

Concentration

daphnids

0h

48h

 

 

 

(mg/l)

 

 

 

 

 

 

 

Control

 

10

21.9

 

21.9

 

 

0.6450

 

10

21.9

 

21.9

 

 

0.6450

 

10

21.9

 

21.9

Validity criteria fulfilled:
yes
Remarks:
1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels
Conclusions:
Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia was determine to be > 0.6450 mg/L.
Executive summary:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test chemical . The test substance was partially soluble in water. Therefore, the stock solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring.After stirring the stock solution was analytically detected and the stock concentration was found to be 0.6450 mg/L. Limit test was set up with this concentration only and test Daphnia magna were exposed to these concentration for 48 hours. Test conducted under the semi-static system in which 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml were used. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia was determine to be > 0.6450 mg/L. Test consider to be valid as fullfils all the criteria as per standards. 1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized.  2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. As the chemical was slightly soluble in water and test conducted at higher solubility value i.e. > 0.6450 mg/l at which no mobility were observed, thus on that basis chemical cannot be consider to be toxic and not classified as per the CLP classification criteria.

Description of key information

Short term toxicity to aquatic invertebrate:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test chemical . The test substance was partially soluble in water. Therefore, the stock solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring.After stirring the stock solution was analytically detected and the stock concentration was found to be 0.6450 mg/L. Limit test was set up with this concentration only and test Daphnia magna were exposed to these concentration for 48 hours. Test conducted under the semi-static system in which 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml were used. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia was determine to be > 0.6450 mg/L. Test consider to be valid as fullfils all the criteria as per standards. 1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized.  2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. As the chemical was slightly soluble in water and test conducted at higher solubility value i.e. > 0.6450 mg/l at which no mobility were observed, thus on that basis chemical cannot be consider to be toxic and not classified as per the CLP classification criteria.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.645 mg/L

Additional information

Short term toxicity to aquatic invertebrate:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test chemical . The test substance was partially soluble in water. Therefore, the stock solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring.After stirring the stock solution was analytically detected and the stock concentration was found to be 0.6450 mg/L. Limit test was set up with this concentration only and test Daphnia magna were exposed to these concentration for 48 hours. Test conducted under the semi-static system in which 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml were used. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia was determine to be > 0.6450 mg/L. Test consider to be valid as fullfils all the criteria as per standards. 1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized.  2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. As the chemical was slightly soluble in water and test conducted at higher solubility value i.e. > 0.6450 mg/l at which no mobility were observed, thus on that basis chemical cannot be consider to be toxic and not classified as per the CLP classification criteria.