2,2'-(vinylenedi-p-phenylene)bisbenzoxazole

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17-05-2018 to 19-05-2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Acute Immobilization study of test material on Daphnia magna in a Semi-Static System for 48 hrs. Test performed according to the OECD guideline 202.

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

The test substance was partially soluble in water. Therefore, the stock solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring.After stirring the stock solution was analytically detected and the stock concentration was found to be 0.6450 mg/L. Limit test was set up with this concentration only.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

48 h

Post exposure observation period:

24 and 48 hrs

Hardness:

150.5 mg of CaCO3

Test temperature:

20±2 degree C

pH:

7.4

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.645 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Other details not known

9. OBSERVATIONS:
9.1. Immobility
Nominal		Number	of	Immobility
Concentration		Daphnids	0 h	24 h			48 h
(mg/l)
Control		10	-	-			-
0.6450		10	-	-			-
0.6450		10	-	-			-
Any	Abnormal			-	No observed effects.		No observed effects.
Behaviour (if any)
9.2. pH and oxygen content:
		Nominal		Number of	pH(mg/L)
		Concentration		daphnids	0h	48h
		(mg/l)
		Control		10	7.1	7.2	7.2
		0.6450		10	7.3	7.4	7.4
		0.6450		10	7.3	7.4	7.4
		Nominal		Number of	DO(mg/L)
		Concentration		daphnids	0h	48h
		(mg/l)
		Control		10	6.9	6.9	6.9
		0.6450		10	6.8	6.8	6.8
9.3. Test temperature		0.6450		10	6.8	6.8	6.8
		Nominal		Number of	Temparature
		Concentration		daphnids	0h	48h
		(mg/l)
		Control		10	21.9		21.9
		0.6450		10	21.9		21.9
		0.6450		10	21.9		21.9

Validity criteria fulfilled:

yes

Remarks:

1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels

Conclusions:

Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia was determine to be > 0.6450 mg/L.

Executive summary:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test chemical . The test substance was partially soluble in water. Therefore, the stock solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring.After stirring the stock solution was analytically detected and the stock concentration was found to be 0.6450 mg/L. Limit test was set up with this concentration only and test Daphnia magna were exposed to these concentration for 48 hours. Test conducted under the semi-static system in which 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml were used. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia was determine to be > 0.6450 mg/L. Test consider to be valid as fullfils all the criteria as per standards. 1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized.  2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. As the chemical was slightly soluble in water and test conducted at higher solubility value i.e. > 0.6450 mg/l at which no mobility were observed, thus on that basis chemical cannot be consider to be toxic and not classified as per the CLP classification criteria.

Description of key information

Short term toxicity to aquatic invertebrate:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test chemical . The test substance was partially soluble in water. Therefore, the stock solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring.After stirring the stock solution was analytically detected and the stock concentration was found to be 0.6450 mg/L. Limit test was set up with this concentration only and test Daphnia magna were exposed to these concentration for 48 hours. Test conducted under the semi-static system in which 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml were used. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia was determine to be > 0.6450 mg/L. Test consider to be valid as fullfils all the criteria as per standards. 1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized.  2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. As the chemical was slightly soluble in water and test conducted at higher solubility value i.e. > 0.6450 mg/l at which no mobility were observed, thus on that basis chemical cannot be consider to be toxic and not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.645 mg/L

Additional information

Short term toxicity to aquatic invertebrate:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test chemical . The test substance was partially soluble in water. Therefore, the stock solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring.After stirring the stock solution was analytically detected and the stock concentration was found to be 0.6450 mg/L. Limit test was set up with this concentration only and test Daphnia magna were exposed to these concentration for 48 hours. Test conducted under the semi-static system in which 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml were used. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia was determine to be > 0.6450 mg/L. Test consider to be valid as fullfils all the criteria as per standards. 1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized.  2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. As the chemical was slightly soluble in water and test conducted at higher solubility value i.e. > 0.6450 mg/l at which no mobility were observed, thus on that basis chemical cannot be consider to be toxic and not classified as per the CLP classification criteria.