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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15-05-2018 to 19-05-2018
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
not specified
Analytical monitoring:
yes
Vehicle:
not specified
Details on test solutions:
The test substance was partially soluble in RO water. Therefore, the stock solution was prepared by dissolving 4 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 72 hrs stirring, After stirring the stock solution was analytically detected and the stock concentration was found to be 0.7309 mg/l, On the basis of this value limit test was set up with this concentration 0.7309 mg/L ,respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 1.88 cm
- Weight at study initiation (mean and range, SD): average 0.0792g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
190 mg of CaCO3
Test temperature:
23 °C
pH:
7.2 - 7.9
Dissolved oxygen:
6.9 mg/l
Nominal and measured concentrations:
0.7309 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
0.731 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: No effect were observed
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 0.731 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Other details not known

Fig: 1. Mortality and symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

-

-

-

-

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

pH and oxygen content:

 

 

Number of

pH readings after

 

 

 

 

Nominal

fish

24h

 

48h

 

72h

 

96h

 

Concentration

 

 

V1

V2

V1

V2

V1

V2

V1

V2

(mg/l)

 

 

 

 

 

 

 

 

 

 

Control

4

4

7.2

7.2

7.5

7.5

7.6

7.6

7.7

7.7

0.7309

4

4

7.3

7.3

7.7

7.7

7.8

7.8

7.9

7.9

Nominal

Number of

Oxygen content (mg/L) after

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

6.7

6.7

6.6

6.6

6.5

6.5

6.4

6.4

0.7309

4

4

6.6

6.6

6.5

6.5

6.4

6.4

6.3

6.3

8.3. Test temperature

 

 

 

 

 

 

 

 

Nominal

Number of

Temperature (°C) after

 

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

23.8

23.8

23.9

23.9

24.1

24.1

23.8

23.8

0.7309

4

4

23.8

23.8

23.9

23.9

24.1

24.1

23.8

23.8

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test material on Zebra Fish Danio rerio was determine to be >0.7309 mg/L.
Executive summary:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was partially soluble in RO water. Therefore, the stock solution was prepared by dissolving 4 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 72 hrs stirring, After stirring the stock solution was analytically detected and the stock concentration was found to be 0.7309 mg/l, On the basis of this value limit test was set up with this concentration 0.7309 mg/L ,respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Test consider to be valid as all the conditions available according to the OECD guidelines. The mortality in the control not exceed 0 per cent at the end of the test. The constant conditions maintained as far as possible throughout the test and, semi-static procedures used. The dissolved oxygen concentration >60 per cent of the air saturation value throughout the test. After 96 hours of exposure to test item to limit concentrations, LC50 was determine to be > 0.7309 mg/l . As the chemical was slightly soluble in water and mortality was observed to be > 0.7309 mg/l, thus on that basis chemical cannot be consider to be toxic and not classified as per the CLP classification criteria.

Description of key information

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was partially soluble in RO water. Therefore, the stock solution was prepared by dissolving 4 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 72 hrs stirring, After stirring the stock solution was analytically detected and the stock concentration was found to be 0.7309 mg/l, On the basis of this value limit test was set up with this concentration 0.7309 mg/L ,respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Test consider to be valid as all the conditions available according to the OECD guidelines. The mortality in the control not exceed 0 per cent at the end of the test. The constant conditions maintained as far as possible throughout the test and, semi-static procedures used. The dissolved oxygen concentration >60 per cent of the air saturation value throughout the test. After 96 hours of exposure to test item to limit concentrations, LC50 was determine to be > 0.7309 mg/l. As the chemical was slightly soluble in water and mortality was observed to be > 0.7309 mg/l, thus on that basis chemical cannot be consider to be toxic and not classified as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
0.731 mg/L

Additional information

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was partially soluble in RO water. Therefore, the stock solution was prepared by dissolving 4 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 72 hrs stirring, After stirring the stock solution was analytically detected and the stock concentration was found to be 0.7309 mg/l, On the basis of this value limit test was set up with this concentration 0.7309 mg/L ,respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Test consider to be valid as all the conditions available according to the OECD guidelines. The mortality in the control not exceed 0 per cent at the end of the test. The constant conditions maintained as far as possible throughout the test and, semi-static procedures used. The dissolved oxygen concentration >60 per cent of the air saturation value throughout the test. After 96 hours of exposure to test item to limit concentrations, LC50 was determine to be > 0.7309 mg/l. As the chemical was slightly soluble in water and mortality was observed to be > 0.7309 mg/l, thus on that basis chemical cannot be consider to be toxic and not classified as per the CLP classification criteria.