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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
Experimental data of read across chemicals
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
other: As mention in below principle
Principles of method if other than guideline:
WoE report is prepared based on toxicity to microorganisms study:
1 and 2nd
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Photobacterium phosphoreum
Details on inoculum:
1 and 2nd study: Species: Photobacterium phosphoreum commonly known as Vibrio fischerii
Strain: NRRL-B-11177
Test type:
not specified
Water media type:
freshwater
Total exposure duration:
30 min
Post exposure observation period:
5, 15 and 30 minutes
Test temperature:
15°C
pH:
5-9
Nominal and measured concentrations:
Measured
Reference substance (positive control):
yes
Remarks:
Most frequently used and recommended reference chemicals are phenol and sodium pentachlorophenate
Key result
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
3.35 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: toxicity to luminescent bacteria
Remarks on result:
other: 1st study
Key result
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
5.43 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: toxicity to luminescent bacteria
Remarks on result:
other: 2nd study

1. study

- pT -Measured concentrations (in log(L/mmol) at which there is a 50% reduction of the light emission relative to controls at the stated exposure times of 5, 15 and 30 min

- ppm -The compound's toxicity expressed in parts per million (ppm) or mg/L.

 

Name

5 min- pT

15 min- pT

30 min-pT

ppm

Test chemical

1.76

1.86

1.89

3.35

2. study:

- pT -Measured concentrations (in log(L/mmol) at which there is a 50% reduction of the light emission relative to controls at the stated exposure times of 5, 15 and 30 min

- ppm -The compound's toxicity expressed in parts per million (ppm) or mg/L.

 

Name

5 min- pT

15 min- pT

30 min-pT

ppm

Test chemical

1.54

1.67

1.71

5.43

Validity criteria fulfilled:
not specified
Conclusions:
1. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 3.35 mg/l after 30 minutes exposure with test chemical.
2. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 5.43 mg/l and 6.83 mg/l after 30 minutes exposure with test chemical.
Thus based on the overall studies, it was concluded that the test chemical was toxic and the toxic concentration ranges from 3.35 mg/l to 6.83 mg/l.
Executive summary:

Various studies available for the test chemical were reviewed to determine the toxic nature of test material on the growth of microorganisms. The studies are as mentioned below:

The comparatively quick and low cost bioassay with Photobacterium phosphoreum, strain NRRL-B-11177 to access the toxicity of test chemical to aquatic bacteria. The luminescent bacterial bioassay requires only a short period of time, i.e. minutes. In terms of testing a broad range of different chemicals with many different modes of action, a somewhat longer assay time, i.e. 30 min, is preferred, mainly to ascertain that potential diffusion problems are eliminated at source. Normally the pT values for a particular chemical rarely vary much with exposure time. The most commonly used operating conditions are 15°C for the EC50 tests with exposure times of 5 to 30 min. The pH of the test system can vary in the range of 5< pH <9 without great effect on the luminescence. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 3.35 mg/l after 30 minutes exposure with test chemical.

Similarly in the second study the comparatively quick and low cost bioassay with Photobacterium phosphoreum, strain NRRL-B-11177 to access the toxicity of test chemical to aquatic bacteria. The luminescent bacterial bioassay requires only a short period of time, i.e. minutes. In terms of testing a broad range of different chemicals with many different modes of action, a somewhat longer assay time, i.e. 30 min, is preferred, mainly to ascertain that potential diffusion problems are eliminated at source. Normally the pT values for a particular chemical rarely vary much with exposure time. The most commonly used operating conditions are 15°C for the EC50 tests with exposure times of 5 to 30 min. The pH of the test system can vary in the range of 5< pH <9 without great effect on the luminescence. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 5.43 mg/l and 6.83 mg/l after 30 minutes exposure with test chemical.

Thus based on the overall studies, it was concluded that the test chemical was toxic and the toxic concentration ranges from 3.35 mg/l to 6.83 mg/l.

Description of key information

1. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 3.35 mg/l after 30 minutes exposure with test chemical.

2. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 5.43 mg/l and 6.83 mg/l after 30 minutes exposure with test chemical.

Thus based on the overall studies, it was concluded that the test chemical was toxic and the toxic concentration ranges from 3.35 mg/l to 6.83 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:
6.83 mg/L

Additional information

Various studies available for the test chemical were reviewed to determine the toxic nature of 2,2'-(ethene-1,2-diyldi-4,1-phenylene)bis(1,3-benzoxazole) (CAS no. 1533 -45 -5) on the growth of microorganisms. The studies are as mentioned below:

 

The comparatively quick and low cost bioassay with Photobacterium phosphoreum, strain NRRL-B-11177 to access the toxicity of test chemical to aquatic bacteria. The luminescent bacterial bioassay requires only a short period of time, i.e. minutes. In terms of testing a broad range of different chemicals with many different modes of action, a somewhat longer assay time, i.e. 30 min, is preferred, mainly to ascertain that potential diffusion problems are eliminated at source. Normally the pT values for a particular chemical rarely vary much with exposure time. The most commonly used operating conditions are 15°C for the EC50 tests with exposure times of 5 to 30 min. The pH of the test system can vary in the range of 5< pH <9 without great effect on the luminescence. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 3.35 mg/l after 30 minutes exposure with test chemical.

 

 

Similarly in the second study the comparatively quick and low cost bioassay with Photobacterium phosphoreum, strain NRRL-B-11177 to access the toxicity of test chemical to aquatic bacteria. The luminescent bacterial bioassay requires only a short period of time, i.e. minutes. In terms of testing a broad range of different chemicals with many different modes of action, a somewhat longer assay time, i.e. 30 min, is preferred, mainly to ascertain that potential diffusion problems are eliminated at source. Normally the pT values for a particular chemical rarely vary much with exposure time. The most commonly used operating conditions are 15°C for the EC50 tests with exposure times of 5 to 30 min. The pH of the test system can vary in the range of 5< pH <9 without great effect on the luminescence. The Effective Concentration causing 50% mortality of luminescent bacteria Photobacteria phosphereum was determine to be 5.43 mg/l and 6.83 mg/l after 30 minutes exposure with test chemical.

 

Thus based on the overall studies, it was concluded that the test chemical was toxic and the toxic concentration ranges from 3.35 mg/l to 6.83 mg/l.