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EC number: 208-419-2
CAS number: 527-60-6
Test conc. Nominal product
Test conc. Measured active ingredient**
*All fish in 8.3, 18.2 and 40 mg/L
concentration were immobile within 5 minutes of exposure
** Based on time-weighted measured
values (and interpolated values for concentrations not measured)
to OECD 203 and in compliance with GLP, a fish acute toxicity test was
conducted using rainbow trout (Oncorhynchus mykiss). A range-finding
test was conducted to determine the test concentrations to be used for
the definitive test. Exposure concentrations, one order of magnitude
apart (800, 80, 8, 0.8 and 0.08 mg/L; as test product), and a control
were used. The preliminary estimates of the LC50 for 96-hour rainbow
trout mortality was between 8 and 80 mg/L, as product (or 7 to 70
mg/Lnominal 2,4,6— trimethylphenol). From these results, the following
nominal concentrations were chosen for the definitive test: 40.0, 18.2,
8.3, 3.8, 1.7 and 0.8 mg/L, plus controls. In the definitive rainbow
trout study, the test concentrations that were not analytically verified
were interpolated from the measured test concentrations. All
concentrations were then time-weighted (OECD, 1998b). Based on the
time-weighted concentrations, the 96-hour LC50 for rainbow trout
mortality was 9.7 mg/L (with 95 % confidence limits of 3.7 to 17.2 mg/L).
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