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EC number: 208-419-2
CAS number: 527-60-6
GLP-compliant chromosome aberration test Chinese hamster ovary (CHO)
cells were exposed to the test substance in DMSO with and without
metabolic activation (S9 -mix). The cells were treated for 4 and 20
hours in the non-activated test system and for 4 hours in the S9
activated test system and all cells were harvested at 20 hours after
treatment initiation. Different dosages (up to 200, 300 and 400 µg/mL,
for +S9 mix 4 h, -S9 mix 4 h and -S9 mix 20 h, respectively) were chosen
in the three groups. Cytotoxicity was observed in all three groups at
the highest tested dose. The percentage of cells with structural
aberrations in the test article-treated groups was significantly
increased above that of the solvent control at dose levels 100 and 200
µg/mL in the S9 activated 4 hour exposure group. The precentage of
numerical aberrations was not significantly increased. In
the non-activated 4 -hour exposure group, the
percentage of cells with structural aberrations was significantly above
that of the solvent control at dose level 300 µg/mL. However, this
percentage of 3.0 % was within the historical solvent control and
therefore not considered to be biologically relevant. In the absence of
the S9 mix in the 20 -hour exposure group, the test article did not
increase the frequency of cells with aberrations. Based on these results
the test substance is considered to be mutagenic in the presence of
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