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EC number: 201-304-8
CAS number: 80-73-9
A dose setting test was conducted to set dose levels for the main test
and the counting of the number of reverted colonies was carried out as
well as an observation as to whether there were antibacterial activity
The dose level in the dose setting test was set to 6 levels at a common
ratio of 4, with 500μg/plate being set as the highest dose level. As a
result, regardless of whether or not metabolic activation was carried
out, the test substance did not show any antibacterial activity against
any of the bacterial strains or an increase of more than two times as
large as the number of reverted colonies compared with the negative
(solvent) control. Moreover, the test substance did not show any
sedimentation at any of the dose levels.
Based on the results described above, a total of 5 dose levels at a
common ratio of 2 was set for all bacterial strains, with the highest
dose level being 5000μg/plate, regardless of whether or not metabolic
activation was carried out.
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