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EC number: 201-304-8
CAS number: 80-73-9
Short term toxicity to fish
In a 96 -h acute toxicity study, Ricefish (Oryzias latipes) (5/group) were exposed to the substance at a nominal concentration of 100 mg/L under semi-static conditions (Biotoxtech 2013, report J12480). There were no mortalities or other adverse reactions to exposure in the exposed fish. The 96 -h LC50 and NOEC values were higher than 100 mg/L, based on mortality. The toxicity study is classified as acceptable and fully satisfies the guideline requirement for short-term toxicity to fish.
Long term toxicity to fish
The study is proposed to be waived.
Short-term toxicity to aquatic invertebrates
The 48–h-acute toxicity of the substance to daphnia magna was studied under static conditions (Safepharm 1998, report 458/176). Daphnids were exposed to control and test chemical at a nominal concentration of 100 mg/L for 48h. Mortality/immobilization and sublethal effects were observed daily and at test termination. The 24h-NOEC and 48h-NOEC based on immobilization were both greater or equal to100 mg/L. The EC50 in the daphnia magna immobilisation test is greater than 100mg/L. This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Long-term toxicity to aquatic invertebrates
This study is proposed to be waived.
Toxicity to aquatic algae and cyanobacteria
An algal growth inhibition test was conducted by Safepharm Laboratories Limited, to determine the effect of the test substance on the aquatic environment. The study was conducted in compliance with GLP, and was performed according to EC test guideline C3 (Safepharm 1998, report number 458/175). The biomass increase and growth rate of a culture of algae (Desmodesmus subspicatus) was monitored over a period of 72 hours in growth media containing test substance at a concentration of 100 mg/L. The 72 -hour ErC50of the test substance with algae was determined to be greater than 100 mg/L.
Toxicity to aquatic plants other than algae
No data available. No data required.
Toxicity to microorganisms
The effect of the substance on the respiration rate of activated sludge was assessed by the methods detailed in and OECD Test Guideline 209 and in compliance with GLP (HLS 2013, LBM0058).
DMI did not significantly inhibit the respiration rates of the samples of activated sludge. The three-hour EC50 must therefore be >1000 mg/L. The no observed effect concentration was greater 1000 mg/L, the highest concentration tested.
Toxicity to other microorganisms
The substance is not toxic in the aquatic environment.
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